malic enzyme 2 (NAD-dependent malic enzyme, mitochondrial, NAD-ME, malic enzyme 2) (ME2)

Function: - feeds into citric acid cycle - malate from pyruvate - subject to allosteric activation by fumarate - can also use NADP⁺ but is more effective with NAD⁺ (S)-malate + NAD⁺ pyruvate + CO₂ + NADH Cofactor: divalent metal cations, prefers Mg⁺² or Mn+2 Structure: - homotetramer - belongs to the malic enzymes family Compartment: - mitochondrial matrix

General

malic enzyme, malate decarboxylase or malate oxidoreductase mitochondrial protein oligomerizing protein

Properties

SIZE: entity length = 584 aa MW = 65 kD COMPARTMENT: mitochondria MOTIF: binding site SITE: 67-67 FOR-BINDING-OF: Allosteric activator binding site SITE: 91-91 FOR-BINDING-OF: Allosteric activator tyrosine residue {Y112} cofactor-binding site [165-173] COFACTOR-BOUND: NAD MOTIF: binding site SITE: 165-165 FOR-BINDING-OF: Substrate lysine residue {K183} cofactor-binding site [259-259] COFACTOR-BOUND: NAD Important for activity {279-279} cofactor-binding site [311-328] COFACTOR-BOUND: NAD binding site SITE: 421-421 FOR-BINDING-OF: Substrate binding site SITE: 466-466 FOR-BINDING-OF: Substrate

Database Correlations

OMIM 154270 MORBIDMAP 154270 UniProt P23368 PFAM correlations ENZYME 1.1.1.38

References

UniProt :accession P23368