linear ubiquitin chain assembly complex (LUBAC complex)

Function: - formation of polyubiquitin linked head-to-tail Structure: - consists of RBCK1 & RNF31 - LUBAC has a MW of approximative 600 kD suggesting a heteromultimeric assembly of its subunits

Properties

SUBUNITS: RanBP-type & C3HC4-type zinc finger-containing protein 1 MOTIF: Ubiquitin-like {55-119} Zinc finger NAME: Zinc finger SITE: 193-222 EFFECTOR-BOUND: Zn+2 coiled coil {233-261} RING-finger {282-327} EFFECTOR-BOUND: Zn+2 FOR-BINDING-OF: DNA motif Tyr phosphorylation site {Y330} zinc finger B box SITE: 376-411 EFFECTOR-BOUND: Zn+2 RING finger protein 31 MOTIF: binding site SITE: 1-485 FOR-BINDING-OF: polyubiquitin MOTIF: Zinc finger NAME: Zinc finger SITE: 299-329 EFFECTOR-BOUND: Zn+2 Zinc finger NAME: Zinc finger SITE: 350-379 EFFECTOR-BOUND: Zn+2 Zinc finger NAME: Zinc finger SITE: 409-438 EFFECTOR-BOUND: Zn+2 Ser phosphorylation site {S466} RBCK1 interaction {563-616} MOTIF: UBA domain {564-615} RING-finger {699-747} EFFECTOR-BOUND: Zn+2 FOR-BINDING-OF: DNA motif Zinc finger NAME: Zinc finger SITE: 779-841 EFFECTOR-BOUND: Zn+2 Zinc finger NAME: Zinc finger SITE: 853-930 EFFECTOR-BOUND: Zn+2

Components

RanBP-type & C3HC4-type zinc finger-containing protein 1; ubiquitin-conjugating enzyme 7-interacting protein 3; hepatitis B virus X-associated protein 4; HBV-associated factor 4; heme-oxidized IRP2 ubiquitin ligase 1; RING finger protein 54 (RBCK1, C20orf18, RNF54, UBCE7IP3, XAP3, XAP4) RING finger protein 31; HOIL-1-interacting protein; HOIP; Zn+2 in-between-RING-finger ubiquitin-associated domain protein (RNF31, ZIBRA)