LIM domain & actin-binding protein 1 (epithelial protein lost in neoplasm, LIMA1, EPLIN, SREBP3, PP624)

Function: 1) binds to actin monomers & filaments 2) increases number & size of actin stress fibers & inhibits membrane ruffling 3) inhibits actin filament depolymerization 4) bundles actin filaments, delays filament nucleation & reduces formation of branched filaments Structure: - contains 1 LIM zinc-binding domain - contains at least 2 actin-binding domains one on each side of the LIM domain both domains bind actin monomers & filaments the C-terminal domain binds filaments more efficiently than the N-terminus Compartment: - cytoplasm, cell junction, focal adhesion, cytoskeleton - co-localizes with actin stress fibers & focal adhesion plaques Alternative splicing: - named isoforms=3 - alternative promoter usage Expression: - highly expressed in placenta, kidney, pancreas, prostate, ovary, spleen & heart - also detected in lung, liver, brain, skeletal muscle, thymus, testis & intestine - not detected in leukocytes - isoform Beta expressed generally at very low levels, constitutively expressed - isoform Alpha abundant in epithelial cells from mammary gland, prostate & in normal oral keratinocytes induced by serum. - low levels in aortic endothelial cells & dermal fibroblasts - not detectable in myocardium Pathology: - down-regulated in some cancer cell lines

General

cytoskeletal protein phosphoprotein

Properties

SIZE: MW = 85 kD entity length = 759 aa COMPARTMENT: cytoplasm MOTIF: Ser phosphorylation site {S374} LIM domain {388-448} MOTIF: cysteine residue (6) histidine residue Zn+2-binding site Ser phosphorylation site {S490} Ser phosphorylation site {S604} Tyr phosphorylation site {Y752}

Database Correlations

OMIM 608364 UniProt Q9UHB6 Pfam PF00412

References

UniProt :accession Q9UHB6