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leucine-rich repeat-containing protein 16A (CARMIL homolog, LRRC16A, CARMIL, LRRC16)
Function:
- binds CAPZA2 with high affinity & significantly decreases CAPZA2 affinity for actin barbed ends
- increases the rate of elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments
- rapidly uncaps barbed ends
- capped by CAPZA2 & enhances barbed-end actin polymerization
- interacts via its C-terminus with CAPZA2 in cytoplasm
- interaction is essential for lamellipodial protrusion & cell translocation
Structure:
- belongs to the CARMIL family
- contains 11 LRR repeats (leucine-rich repeats)
Compartment:
- cytoplasm
- colocalized with F-actin in lamellipodia but not with F-actin in stress fibers
Alternative splicing: named isoforms=4
Expression:
- expressed in lung, placenta, small intestine, liver, thymus, colon, skeletal muscle, heart & brain
- higher expression in kidney
General
cytoskeletal protein
phosphoprotein
Properties
SIZE: entity length = 1371 aa
MW = 152 kD
COMPARTMENT: cytoplasm
MOTIF: leucine-rich repeat
SITE: 245-269
MOTIF: leucine residue (SEVERAL)
leucine-rich repeat
SITE: 275-298
MOTIF: leucine residue (SEVERAL)
leucine-rich repeat
SITE: 304-327
MOTIF: leucine residue (SEVERAL)
leucine-rich repeat
SITE: 336-363
MOTIF: leucine residue (SEVERAL)
leucine-rich repeat
SITE: 391-418
MOTIF: leucine residue (SEVERAL)
leucine-rich repeat
SITE: 423-447
MOTIF: leucine residue (SEVERAL)
leucine-rich repeat
SITE: 481-506
MOTIF: leucine residue (SEVERAL)
leucine-rich repeat
SITE: 543-566
MOTIF: leucine residue (SEVERAL)
leucine-rich repeat
SITE: 570-593
MOTIF: leucine residue (SEVERAL)
leucine-rich repeat
SITE: 654-678
MOTIF: leucine residue (SEVERAL)
coiled coil {710-734}
Inhibits capping activity of CAPZA2 {958-1082}
MOTIF: leucine-rich repeat
SITE: 958-981
MOTIF: leucine residue (SEVERAL)
Thr phosphorylation site {T1228}
serine-rich region {1243-1250}
MOTIF: serine residue (SEVERAL)
Database Correlations
UniProt Q5VZK9
Pfam PF00560
Kegg hsa:5560
References
UniProt :accession Q5VZK9