lariat debranching enzyme (DBR1)

Function: 1) RNA processing activity that hydrolyzes the 2'-5' phosphodiester linkage at the branchpoint of excised intron lariats (after splicing) - converts them into linear molecules that are subsequently degraded 2) facilitates ribonucleotide turnover Cofactor: divalent metal cations Kinetic parameters: Optimum pH is7.0 Compartment: nucleus Alternative splicing: named isoforms=2 Pathology: 1) retrovirus replication via a RNA lariat intermediate in cDNA synthesis 2) cells lacking DBR1 show an inhibition of HIV-1 replication

General

hydrolase nuclear protein

Properties

SIZE: MW = 62 kD entity length = 544 aa COMPARTMENT: cell nucleus MOTIF: active site

Database Correlations

OMIM 607024 UniProt Q9UK59 PFAM correlations

References

UniProt :accession Q9UK59