kynurenine/alpha-aminoadipate aminotransferase, mitochondrial; KAT/AadAT; 2-aminoadipate aminotransferase; 2-aminoadipate transaminase; alpha-aminoadipate aminotransferase; AadAT; kynurenine aminotransferase 2; kynurenine-oxoglutarate transaminase 2 (AADAT, KAT2)

Function: 1) lysine degradation via saccharopine pathway - glutaryl-CoA from L-lysine: step 4/6 1) converts L-kynurenine to 4-(2-aminophenyl)-2,4-dioxobutanoate 2) converts L-2-aminoadipate to 2-oxoadipate 3) also shows activity also towards tryptophan, aspartate & hydroxykinurenine 4) homodimer ,L-kynurenine + 2-oxoglutarate 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate L-2-aminoadipate + 2-oxoglutarate 2-oxoadipate + L-glutamate Cofactor: pyridoxal phosphate Inhibition: - kynurenine transaminase activity is competitively inhibited by aminoadipate, asparagine, glutamate, histidine, cysteine, lysine, 3-hydroxy-kynurenine & phenylalanine Kinetic parameters: - KM=0.9 mM for aminoadipate - KM=4.7 mM for kynurenine - KM=1.7 mM for methionine - KM=1.6 mM for glutamate - KM=1.8 mM for tyrosine - KM=1.2 mM for 2-oxoglutarate - KM=1.5 mM for 2-oxocaproic acid - KM=1.8 mM for phenylpyruvate - KM=1.4 mM for ino-3-pyruvate - pH dependence: optimum pH is 7-9 - temperature dependence: - optimum temperature is 50 degrees celsius Structure: - homodimer - belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family Compartment: mitochondrion Alternative splicing: named isoforms=2 Expression: - expressed in liver > heart, brain, kidney, pancreas, prostate, testis, ovary

General

aminotransferase mitochondrial protein oligomerizing protein

Properties

SIZE: entity length = 425 aa MW = 47 kD COMPARTMENT: mitochondria MOTIF: binding site SITE: 20-20 FOR-BINDING-OF: Substrate binding site SITE: 74-74 FOR-BINDING-OF: Substrate binding site SITE: 142-142 FOR-BINDING-OF: Substrate binding site SITE: 202-202 FOR-BINDING-OF: Substrate binding site SITE: 399-399 FOR-BINDING-OF: Substrate

Database Correlations

OMIM 611754 UniProt Q8N5Z0 Pfam PF00155 Entrez Gene 51166 Kegg hsa:51166 ENZYME correlations

References

UniProt :accession Q8N5Z0