KIN, antigenic determinant of recA protein homolog (protein KIN homolog, binding to curved DNA, KIN, BTCD, KIN17)

Function: 1) role in DNA replication & cellular response to DNA damage 2) creates a bridge between DNA replication & DNA repair mediated by high molecular weight complexes 3) role in illegitimate recombination & regulation of gene expression 4) role in mRNA processing 5) binds double-stranded DNA in vitro 6) binds preferentially to curved DNA in vitro & in vivo 7) binds to RNA via its C-terminal domain in vitro 8) interacts with SV40 large T antigen 9) associated with DNA polymerase alpha, RFC1 & cyclin A, in multiprotein DNA replication complexes 10) associates with replication origins at G1/S phase boundary & throughout the S phase in vivo Structure: - contains 1 C2H2-type zinc finger - C-terminal domain (268-393) is organized into 2 subdomains that bear structural similarities to SH3-like domains both subdomains adopt a similar 5-stranded beta-barrel-like fold & are connected to each other by a short linker of 5 residues the 5 beta-sheets (folds) are packed at approximately right angles against each other a highly conserved groove formed at the interface between the 2 subdomains, comprised of Lys residues 302 & 391 & other positively charged residues, may possibly be the site of RNA-binding Compartment: - nucleus, cytoplasm - during S phase, strongly associated with the nuclear matrix, & to chromosomal DNA in the presence of DNA damage - cytoplasmic localization in elongated spermatids Expression: - ubiquitously expressed in all tissues examined - highest levels in skeletal muscle, heart, testis - highly expressed in proliferating epithelial keratinocytes in vitro - induced by UVC irradiation in quiescent primary fibroblasts & by mitomycin C in human melanoma MeWO cells Pathology: - recognized by antibodies directed against the RecA protein - differentially expressed in non-tumorigenic & tumorigenic cell lines

General

C2H2 type zinc finger protein DNA-binding protein

Properties

SIZE: MW = 45 kD entity length = 393 aa COMPARTMENT: cytoplasm cell nucleus MOTIF: Zn finger C2H2-type SITE: 28-50 EFFECTOR-BOUND: Zn+2 coiled coil {147-180} coiled coil {250-277} subdomain A {284-334} subdomain B {340-391}

Database Correlations

OMIM 601720 UniProt O60870

References

UniProt :accession O60870