katanin p60 ATPase-containing subunit A1; katanin p60 subunit A1; p60 katanin (KATNA1)

Function: 1) severs microtubules in vitro (ATP-dependent) 2) rapid reorganization of cellular microtubule arrays (putative) a) disassembly of interphase microtubules at G2-M transition b) microtubule release from the centrosome after nucleation c) depolymerization of microtubule end of mitotic spindles, proximal to centrosome, & subsequent poleward microtubule flux 3) in neurons, microtubule release within cell body may allow their subsequent transport into axons by microtubule dependent motor proteins; this transport is required for axonal growth 4) ATPase activity is stimulated by microtubules, which promote homooligomerization 5) ATP dependent microtubule severing is stimulated by interaction KATNB1 6) homooligomerizes into hexameric rings, promoted by microtubules 7) interacts with KATNB1, targeting subunit (putative) 8) phosphorylation by DYRK2 triggers ubiquitination & subsequent degradation 9) ubiquitinated by the EDVP E3 ligase complex & subsequently targeted to proteasomal degradation 10) associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 & VPRBP proteins (EDVP complex) Structure: - the N-terminus is sufficient for interaction with microtubules, although high affinity binding requires an intact C-terminal domain & ATP, which promotes oligomerization - belongs to the AAA ATPase family Compartment: - cytoplasm, cytoskeleton, centrosome, spindle pole - predominantly cytoplasmic - also localized to the interphase centrosome - recruitment to the mitotic spindle poles requires microtubules & interaction with KATNB1 Alternative splicing: named isoforms=2

General

adenosine triphosphatase (ATPase) enzyme regulatory subunit oligomerizing protein

Properties

SIZE: entity length = 491 aa MW = 56 kD COMPARTMENT: cytoplasm MOTIF: microtubule interaction {1-185} MOTIF: KATNB1 interaction {1-29} Ser phosphorylation site {S42} Ser phosphorylation site {S109} Thr phosphorylation site {T133} Ser phosphorylation site {S170} ATP-binding site NAME: ATP-binding site SITE: 249-256

Database Correlations

OMIM 606696 UniProt O75449 PFAM correlations Entrez Gene 11104 Kegg hsa:11104 ENZYME 3.6.4.3

References

UniProt :accession O75449