CD49f (integrin alpha-6, integrin alpha-F, ITGA6)

Function: - associates with integrin beta-1 (integrin alpha-6/beta-1) - associates with integrin beta-4 (integrin alpha-6/beta-4) - interacts with HPS5 - isoforms containing segment A, but not segment B, are major targets for PMA-induced phosphorylation - phosphorylation occurs on Ser-1103 of isoform alpha-6X1X2A - phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand Structure: - composed of an heavy & a light chain linked by a disulfide bond - belongs to the integrin alpha chain family - contains 7 FG-GAP repeats Compartment: - membrane; single-pass type 1 membrane protein Alternative splicing: - named isoforms=6 - additional isoforms seem to exist - at least four alternatively spliced domains, two extracellular (X1 & X2) & two cytoplasmic (A & B) - isoform alpha-6X1A, isoform alpha-6X1B & isoform alpha-6X1X2A (minor) have been detected Expression: - isoforms containing segment X1 are ubiquitously expressed - isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts & myotubes, & in a limited number of cell lines; always coexpressed with the ubiquitous isoform containing segment X1 - in some tissues (e.g. salivary gland), isoforms containing cytoplasmic segment A & isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis & segment B in kidney) Pathology: - defects in ITGA6 are a cause of epidermolysis bullosa with pyloric atresia

General

cluster-of-differentiation antigen; cluster designation antigen; CD antigen integrin-alpha

Properties

SIZE: entity length = 1130 aa MW = 127 kD COMPARTMENT: cellular membrane MOTIF: signal sequence {1-23} FG-GAP {42-79} MOTIF: N-glycosylation site {N78} cysteine residue {C86} MODIFICATION: cysteine residue {C94} cysteine residue {C94} MODIFICATION: cysteine residue {C86} FG-GAP {113-145} MOTIF: cysteine residue {C131} MODIFICATION: cysteine residue {C154} cysteine residue {C154} MODIFICATION: cysteine residue {C131} cysteine residue {C175} MODIFICATION: cysteine residue {C188} FG-GAP {185-217} MOTIF: cysteine residue {C188} MODIFICATION: cysteine residue {C175} N-glycosylation site {N223} FG-GAP {295-331} MOTIF: N-glycosylation site {N323} FG-GAP {353-391} MOTIF: Ca+2-binding site SITE: 363-371 N-glycosylation site {N409} FG-GAP {414-450} MOTIF: Ca+2-binding site SITE: 425-433 FG-GAP {469-509} MOTIF: Ca+2-binding site SITE: 480-488 cysteine residue {C528} MODIFICATION: cysteine residue {C535} cysteine residue {C535} MODIFICATION: cysteine residue {C528} cysteine residue {C541} MODIFICATION: cysteine residue {C601} kxgffkr NAME: kxgffkr SITE: cytoplasmic domain FOR-BINDING-OF: calreticulin FORM: kxgffkr cysteine residue {C601} MODIFICATION: cysteine residue {C541} cysteine residue {C665} MODIFICATION: cysteine residue {C671} cysteine residue {C671} MODIFICATION: cysteine residue {C665} cysteine residue {C765} MODIFICATION: cysteine residue {C776} N-glycosylation site {N770} cysteine residue {C776} MODIFICATION: cysteine residue {C765} N-glycosylation site {N787} cysteine residue {C920} MODIFICATION: cysteine residue {C-INTERCHAIN} N-glycosylation site {N930} N-glycosylation site {N966} cysteine residue {C973} MODIFICATION: cysteine residue {C978} cysteine residue {C978} MODIFICATION: cysteine residue {C973} N-glycosylation site {N997} transmembrane domain {1051-1076} HPS5 interaction {1077-1083} MOTIF: GFFKR {1079-1083}

Database Correlations

OMIM correlations UniProt P23229 PFAM correlations Entrez Gene 3655 Kegg hsa:3655