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immature colon carcinoma transcript 1 protein; peptidyl-tRNA hydrolase ICT1, mitochondrial; digestion substraction 1; DS-1 (ICT1, DS1)

Function: - peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit - codon-independent translation release factor that has lost all stop codon specificity & directs the termination of translation in mitochondrion, possibly in case of abortive elongation - may be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated & thus in the recycling of stalled mitochondrial ribosomes N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA component of the mitochondrial 39S ribosomal subunit Structure: - belongs to the prokaryotic/mitochondrial release factor family, ICT1 subfamily - in contrast to other members of the family, lacks the regions that come into close contact with the mRNA in the ribosomal A-site & determine the STOP codon specificity, explaining the loss of codon specificity for translation release factor activity Compartment: mitochondria Expression: - down-regulated during the in vitro differentiation of HT29-D4 colon carcinoma cells

General

hydrolase mitochondrial protein phosphoprotein

Properties

SIZE: entity length = 206 aa MW = 24 kD COMPARTMENT: mitochondria MOTIF: Thr phosphorylation site {T169}

Database Correlations

OMIM 603000 UniProt Q14197 Pfam PF00472 Entrez Gene 3396 Kegg hsa:3396 ENZYME 3.1.1.29

References

UniProt :accession Q14197