hydroxylysine kinase; 5-hydroxy-L-lysine kinase; aminoglycoside phosphotransferase domain-containing protein 1 (HYKK, AGPHD1)

Function: - catalyzes GTP-dependent phosphorylation of 5-hydroxy-L-lysine GTP + 5-hydroxy-L-lysine = GDP + 5-phosphonooxy-L-lysine Kinetic parameters: - KM=280 uM for (5R)-5-hydroxy-L-lysine (2 mM ATP) - KM=7.6 uM for (5R)-5-hydroxy-L-lysine (0.2 mM GTP) - KM=810 uM for ATP (0.5 mM (5R)-5-hydroxy-L-lysine) - KM=3.5 uM for GTP (0.5 mM (5R)-5-hydroxy-L-lysine) - Vmax=4.95 umol/min/mg enzyme toward (5R)-5-hydroxy-L-lysine (2 mM ATP) - Vmax=0.49 umol/min/mg enzyme toward (5R)-5-hydroxy-L-lysine (0.2 mM GTP) - Vmax=5.28 umol/min/mg enzyme toward ATP (0.5 mM (5R)-5-hydroxy-L-lysine) - Vmax=0.57 umol/min/mg enzyme toward GTP (0.5 mM (5R)-5-hydroxy-L-lysine) Structure: - belongs to the aminoglycoside phosphotransferase family Compartment: cytoplasm (putative) Alternative splicing: named isoforms=3 Note: - variant in position: 165:V->I (in dbSNP:4380026).

Properties

SIZE: entity length = 373 aa MW = 42 kD COMPARTMENT: cytoplasm MOTIF: kinase domain MOTIF: ATP-binding site NAME: ATP-binding site aspartate residue {D226}

Database Correlations

OMIM 614681 UniProt A2RU49 Pfam PF01636 Entrez Gene 123688 Kegg hsa:123688 ENZYME 2.7.1.81

References

UniProt :accession A2RU49