histone-lysine N-methyltransferase SETMAR (SET domain & mariner transposase fusion gene-containing protein, Metnase, Hsmar1, [Includes: Histone-lysine N-methyltransferase; Mariner transposase Hsmar1], SETMAR)

Function: 1) histone methyltransferase 2) methylates Lys-4 & Lys-36 of histone H3, 2 specific tags for epigenetic transcriptional activation 3) mediates dimethylation of H3 Lys-36 4) binds DNA via mariner transposase Hsmar1 region 5) role in non-homologous end-joining repair (putative) 6) no transposase activity because the active site contains an Asn in position 610 instead of a Asp residue S-adenosyl-L-methionine + histone L-lysine S-adenosyl-L-homocysteine + histone 6-N-methyl-L-lysine Structure: - contains 1 post-SET domain - contains 1 pre-SET domain - contains 1 SET domain - mariner transposase region present in primates appeared 40-58 million years ago, after insertion of a transposon downstream of a preexisting SET gene, followed by de novo exonization of previously non-coding sequence & creation of a new intron. - N-terminal section; belongs to the histone-lysine methyltransferase family - C-terminal section; belongs to the mariner transposase family Compartment: nucleus Alternative splicing: named isoforms=2 Expression: - widely expressed - highest expression in placenta & ovary - lowest expression in skeletal muscle

Properties

SIZE: MW = 77 kD entity length = 671 aa COMPARTMENT: cell nucleus MOTIF: Histone-lysine N-methyltransferase {1-332} MOTIF: pre-SET domain {60-123} SET domain {125-254} post-SET domain {270-286} Mariner transposase Hsmar1 {333-671} MOTIF: coiled coil {539-569}

Database Correlations

UniProt Q53H47 PFAM correlations ENZYME 2.1.1.43

References

UniProt :accession Q53H47