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histone-lysine N-methyltransferase, H3 lysine-79 specific; DOT1-like protein; histone H3-K79 methyltransferase; H3-K79-HMTase; lysine N-methyltransferase 4 (DOT1L, KIAA1814, KMT4)
Function:
- histone N-methyltransferase
- methylates Lys-79 of histone H3
- nucleosomes are preferred as substrate compared to free histones
- binds to DNA
- interacts with MLLT10
S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]
Structure:
- belongs to the DOT1 family
- in contrast to other lysine histone N-methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of Lys of histones
Compartment: nucleus (probable)
Alternative splicing: named isoforms=2
General
nuclear protein
N-methyltransferase
Properties
SIZE: entity length = 1739 aa
MW = 185 kD
COMPARTMENT: cell nucleus
MOTIF: binding site
SITE: 139-139
FOR-BINDING-OF: S-adenosylmethionine
SAM-binding motif 1 {157-168}
MOTIF: S-adenosyl-L-methionine binding {161-163}
binding site
SITE: 168-168
FOR-BINDING-OF: S-adenosylmethionine
SAM-binding motif 2 {183-189}
MOTIF: binding site
SITE: 186-186
FOR-BINDING-OF: S-adenosylmethionine
binding site
SITE: 222-222
FOR-BINDING-OF: S-adenosylmethionine
SAM-binding motif 3 {236-245}
Ser phosphorylation site {S297}
Ser phosphorylation site {S374}
nucleosomes and DNA interaction {391-416}
Ser phosphorylation site {S471}
Thr phosphorylation site {T480}
Ser phosphorylation site {S596}
Ser phosphorylation site {S775}
Ser phosphorylation site {S822}
Ser phosphorylation site {S826}
Ser phosphorylation site {S834}
Ser phosphorylation site {S899}
Thr phosphorylation site {T900}
Ser phosphorylation site {S902}
Ser phosphorylation site {S1001}
Ser phosphorylation site {S1009}
Ser phosphorylation site {S1104}
Ser phosphorylation site {S1246}
Ser phosphorylation site {S1349}
Database Correlations
OMIM 607375
UniProt Q8TEK3
Pfam PF08123
Entrez Gene 84444
Kegg hsa:84444
ENZYME 2.1.1.43
References
UniProt :accession Q8TEK3