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heparin neutralization; heparin removal (Hepzyme)
Indications:
- ruling out heparin contamination as a cause of abnormal coagulation tests, i.e. PT, aPTT etc
- useful for removing heparin from blood specimens drawn from a heparinized line
- useful for assessing liver function in patients on heparin therapy
Principle:
- platelet-poor plasma is added to a vial of heparanase (Hepzyme)
- heparanase cleaves heparin to produce oligosaccharides without antithrombotic activity
- Hepzyme can neutralize up to 2 USP units of unfractionated heparin in 1 ml of citrated plasma
General
specimen processing
References
- Laboratory Service Manual
Children's Hospital and Clinics of Minnesota
http://www.childrensmn.org/Manuals/Lab/Coagulation/019122.pdf
- Heparin Neutralization
University of Texas
http://www.utmb.edu/lsg/hem/HepNeut.htm