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HA2/AP2 adaptor complex

Alias clathrin assembly protein [AP]-2 Function: - specifically recognizes or associates vesicles with the plasma membrane. - role in protein sorting in the trans-Golgi network (TGN) &/or endosomes - seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface - the complex binds polyphosphoinositides Structure: - heterotetramer of a) an alpha-type (AP2A1 or AP2A2) b) a beta type (AP2B1) c) a mu-type (AP2M1) d) a sigma type (AP2S1)

General

HA/AP adaptor complex

Properties

COMPARTMENT: clathrin coated vesicle MOTIF: ligand-binding site SUBUNITS: AP-2 complex subunit beta-1 MOTIF: proline-rich region SITE: 576-716 MOTIF: proline residue (SEVERAL) Tyr phosphorylation site {Y737} Tyr phosphorylation site {Y888} alpha adaptin MOTIF: membrane region AP-2 complex subunit mu-1 MOTIF: Thr phosphorylation site {T156} MHD {167-435} MOTIF: binding site SITE: 341-341 FOR-BINDING-OF: phosphatidylinositol binding site SITE: 343-343 FOR-BINDING-OF: phosphatidylinositol binding site SITE: 345-345 FOR-BINDING-OF: phosphatidylinositol binding site SITE: 354-354 FOR-BINDING-OF: phosphatidylinositol binding site SITE: 356-356 FOR-BINDING-OF: phosphatidylinositol AP-2 complex subunit sigma-1 MOTIF: membrane region

References

  1. Serafini T et al A coat subunit of Golgi-derived non-clathrin-coated vesicles with homology to the clathrin-coated vesicle coat protein beta-adaptin. Nature. 1991 Jan 17;349(6306):215-20. PMID: 1898984
  2. Morris SA, Mann A, Ungewickell E. Analysis of 100-180-kDa phosphoproteins in clathrin-coated vesicles from bovine brain. J Biol Chem. 1990 Feb 25;265(6):3354-7. PMID: 1968063

Components

alpha adaptin (adaptor-related protein complex 2, alpha subunit, clathrin adaptor complex AP2, alpha subunit) AP-2 complex subunit beta-1; adapter-related protein complex 2 beta-1 subunit; beta2-adaptin; beta-adaptin; plasma membrane adaptor HA2/AP2 adaptin beta subunit; clathrin assembly protein complex 2 beta large chain; AP105B (AP2B1, ADTB2, CLAPB1) AP-2 complex subunit mu-1; adaptor protein 50; adaptin-mu2; AP-2 mu-2 chain; plasma membrane adaptor AP-2 50 kD protein; HA2 50 kD subunit; clathrin assembly protein complex 2 medium chain; clathrin coat assembly protein AP50; clathrin coat-associated protein AP50 (AP2M1, CLAPM1, KIAA0109) AP-2 complex subunit sigma-1; adaptor protein 17; adaptor-related protein complex 2 sigma-1 subunit; sigma2-adaptin; plasma membrane adaptor AP-2 17 kD protein; HA2 17 kD subunit; clathrin assembly protein 2 small chain; clathrin coat assembly protein AP17; clathrin coat-associated protein AP17 (AP2S1, AP17, CLAPS2)