guanine nucleotide exchange factor GEFT; RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT, Rac/Cdc42/Rho exchange factor GEFT; p63RhoGEF (GEFT, ARHGEF25)

Function: - may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers - works as a guanine nucleotide exchange factor for Rho family of small GTPases - links specifically G alpha q/11-coupled receptors to RHOA activation - may be an important regulator of processes involved in axon & dendrite formation. - in neurons seems to be an exchange factor primarily for RAC1 - involved in skeletal myogenesis (putative) - interacts with activated GNAQ & GNA11 - interacts with RHOA, CDC42 & RAC1 Structure: - guanine nucleotide exchange activity is autoinhibited by the PH domain - contains 1 DH (DBL-homology) domain - contains 1 PH domain Compartment: - cytoplasm, myofibril, sarcomer - highly colocalizes with actin regions Alternative splicing: named isoforms=2 Expression: - isoforms 1 & 2 are highly expressed in excitable tissues, such as brain, heart & muscle - detected in kidney & liver

Properties

SIZE: entity length = 580 aa MW = 64 kD COMPARTMENT: cytoplasm MOTIF: Dbl homology domain {160-336} MOTIF: Important for binding to Rho GTPases {278-299} PH domain {348-466} Sufficient to bind activated GNAQ {467-493}

Database Correlations

UniProt Q86VW2 Pfam PF00621 Kegg hsa:1155

References

UniProt :accession Q86VW2