guanine nucleotide exchange factor DBS; DBL's big sister; MCF2-transforming sequence-like protein (MCF2L, KIAA0362)

Function: - guanine nucleotide exchange factor - potentially links pathways that signal through RAC1, RHOA & CDC42 - catalyzes guanine nucleotide exchange on RHOA & CDC42 & interacts specifically with the GTP-bound form of RAC1, suggesting that it functions as an effector of RAC1 may also participate in axonal transport in the brain - becomes activated & highly tumorigenic by truncation of the N-terminus (putative) - isoform 5 activates CDC42 - interacts with CCPG1, which results in specific inhibition of its exchange activity toward RHOA (putative) - interacts with an array of inositol phospholipids such as - phosphatidylinositol 3-phosphate (PI3P) - phosphatidylinositol 4-phosphate (PI4P) - phosphatidylinositol 5-phosphate (PI5P) Structure: - the CRAL-TRIO domain is involved in interaction with inositol phospholipids - the DH domain is involved in interaction with CCPG1 - belongs to the MCF2 family - contains 1 CRAL-TRIO domain - contains 1 DH (DBL-homology) domain - contains 1 PH domain - contains 1 SH3 domain - contains 1 spectrin repeat Compartment: - cytoplasm (putative) - plasma membrane, cytoplasmic side (putative) Alternative splicing: named isoforms=8

General

guanine nucleotide exchange factor (GDP/GTP dissociation stimulator, guanine nucleotide-releasing factor/protein, GNEF, GNDS, GDS) phosphoprotein

Properties

SIZE: entity length = 1137 aa MW = 128 kD COMPARTMENT: cytoplasm MOTIF: CRAL-TRIO lipid binding domain {52-224} spectrin repeat {351-456} coiled coil {503-529} Dbl homology domain {631-811} PH domain {829-945} Ser phosphorylation site {S975} src homology 3 [SH3] domain SITE: 1055-1116

Database Correlations

OMIM 609499 UniProt O15068 PFAM correlations Entrez Gene 23263 Kegg hsa:23263

References

UniProt :accession O15068