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group 3 secretory phospholipase A2; group 3 secretory phospholipase A2; GIII sPLA2; sPLA2-III; phosphatidylcholine 2-acylhydrolase 3 (PLA2G3)
Function:
- catalyzes Ca+2-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides
- shows an 11-fold preference for phosphatidylglycerol over phosphatidylcholine
- role in ciliogenesis
- arachidonic acid release is markedly increased by glypican, a glycosylphosphatidylinositol-anchored heparan sulfate proteoglycan
- N-glycosylation does not affect the catalytical activity, but is required for proper secretion
- a nonglycosylated form is found in several cell types
- in several cell types, the N- & C-termini are cleaved off
Cofactor: binds 1 Ca+2 per subunit
Structure:
- belongs to the phospholipase A2 family
Compartment:
- secreted, cell membrane, cytoplasm,cytoskeleton, centrosome, centriole
Expression:
- expressed in kidney, heart, liver, & skeletal muscle
- also present in placenta & peripheral blood leukocytes
- not detected in brain, colon, thymus, spleen & small intestine
- in lung, expressed in bronchial epithelial cells & alveolar macrophages, but scarcely detected in alveolar epithelium, arterial walls & interstitial fibroblasts (at protein level)
- in joints of osteoarthritis & rheumatoid arthritis, expressed in endothelial cells (at protein level)
- in normal heart, detected in some vessels
- in myocardial tissues with acute infarction, expressed in vascular endothelial cells adjacent to cardiomyocytes & those in lesions with granulation
- expression in cardiomyocytes is scarce (at protein level)
- in uterus, breast & colon cancers, detected in tumor cells & neighboring microvascular endothelium, but not in normal glandular tissues (at protein level)
- induced by IL1B/interleukin-1 beta & TNF in microvascular endothelial cells (at protein level)
General
Ca+2 binding protein
glycoprotein
phospholipase A2; phosphatidylcholine 2-acylhydrolase (PLA2)
phosphoprotein
secreted protein
Properties
SIZE: entity length = 509 aa
MW = 57 kD
COMPARTMENT: cytoplasm
MOTIF: signal sequence {1-19}
Phospholipase A2-like {150-291}
MOTIF: Ca+2-binding site
SITE: 158-158
cysteine residue {C159}
MODIFICATION: cysteine residue {C181}
Ca+2-binding site
SITE: 160-160
Ca+2-binding site
SITE: 162-162
N-glycosylation site {N167}
cysteine residue {C180}
MODIFICATION: cysteine residue {C220}
cysteine residue {C181}
MODIFICATION: cysteine residue {C159}
histidine residue {H184}
Ca+2-binding site
SITE: 185-185
cysteine residue {C187}
MODIFICATION: cysteine residue {C213}
cysteine residue {C211}
MODIFICATION: cysteine residue {C244}
cysteine residue {C213}
MODIFICATION: cysteine residue {C187}
aspartate residue {D214}
cysteine residue {C220}
MODIFICATION: cysteine residue {C180}
cysteine residue {C244}
MODIFICATION: cysteine residue {C211}
N-glycosylation site {N280}
N-glycosylation site {N325}
Thr phosphorylation site {T326}
Thr phosphorylation site {T327}
Ser phosphorylation site {S335}
N-glycosylation site {N396}
N-glycosylation site {N439}
Database Correlations
OMIM 611651
UniProt Q9NZ20
Pfam PF05826
Entrez Gene 50487
Kegg hsa:50487
ENZYME 3.1.1.4
References
UniProt :accession Q9NZ20