Search
A-kinase anchor protein 12; A-kinase anchor protein 250 kDa; AKAP 250; gravin; myasthenia gravis autoantigen (AKAP12, AKAP250)
Function:
- anchoring protein
- mediates compartmentalization of protein kinase A & protein kinase C
- phosphorylated upon DNA damage, probably by ATM or ATR
- binds to dimeric RII-alpha regulatory subunit of PKC
Structure:
- polybasic regions located between residues 266 & 557 are involved in binding PKC
- contains 3 AKAP domains
Compartment:
- cytoplasm, cell cortex (probable)
- cytoplasm, cytoskeleton (probable)
- may be part of the cortical cytoskeleton
Alternative splicing: named isoforms=3
Expression:
- expressed in endothelial cells, cultured fibroblasts & osteosarcoma
- not expressed in platelets, leukocytes, monocytic cell lines or peripherical blood cells
- expression induced by lysophosphatidylcholine (lysoPC)
Pathology:
- antibodies to C terminal portion seen in myasthenia gravis
General
cytoskeletal protein
phosphoprotein
Properties
SIZE: entity length = 1782 aa
MW = 191 kD
COMPARTMENT: cytoplasm
MOTIF: Ser phosphorylation site {S19}
glutamate-rich region {98-101}
MOTIF: glutamate residue (SEVERAL)
Ser phosphorylation site {S219}
Ser phosphorylation site {S253}
binding site
SITE: 266-557
FOR-BINDING-OF: PKC
MOTIF: Ser phosphorylation site {S283}
Ser phosphorylation site {S286}
Ser phosphorylation site {S312}
Tyr phosphorylation site {Y374}
Ser phosphorylation site {S388}
Ser phosphorylation site {S483}
Ser phosphorylation site {S505}
Ser phosphorylation site {S557}
Ser phosphorylation site {S565}
Ser phosphorylation site {S566}
Ser phosphorylation site {S568}
Ser phosphorylation site {S569}
Ser phosphorylation site {S598}
AKAP 1 {604-634}
MOTIF: Ser phosphorylation site {S612}
Ser phosphorylation site {S627}
Ser phosphorylation site {S629}
Ser phosphorylation site {S645}
Thr phosphorylation site {T646}
Ser phosphorylation site {S648}
Ser phosphorylation site {S696}
Ser phosphorylation site {S697}
Ser phosphorylation site {S698}
Ser phosphorylation site {S732}
AKAP 2 {753-783}
AKAP 3 {798-828}
Ser phosphorylation site {S887}
Ser phosphorylation site {S1331}
binding site
SITE: 1541-1554
FOR-BINDING-OF: RII
Database Correlations
OMIM 604698
UniProt Q02952
PFAM correlations
Entrez Gene 9590
References
UniProt :accession Q02952