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granulocyte colony-stimulating factor receptor; G-CSF-R; CD114 (CSF3R, GCSFR)
Function:
- receptor for G-CSF
- regulates myeloid differentiation & proliferation
- also may function in some adhesion or recognition events at the cell surface
Structure:
- dimer (probable)
- the WSXWS motif appears to be necessary for proper protein folding, efficient intracellular transport & cell- surface receptor binding
- the box 1 motif is required for JAK interaction &/or activation
- belongs to the type I cytokine receptor family, type 2 subfamily
- contains 5 fibronectin F3 modules
- contains 1 Ig-like C2-type domain (immunoglobulin-like)
Compartment:
- isoform 2: secreted (probable)
- cell membrane
- isoform 2: secreted (probable)
Alternative splicing:
- named isoforms=4
- additional isoforms seem to exist
Expression:
- granulocytes
- monocytes
- platelets
- endothelium
- placenta
- trophoblastic cells
- one or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U937 cell line, in bone marrow cells, placenta, & peripheral blood granulocytes
- isoform GCSFR-2 is found only in leukemia U937 cells
- isoform GSCFR-3 is highly expressed in placenta
- eosinophils, lymphocytes, erythrocytes stain negatively with antibody
Pathology: -defects in CSF3R may be a cause of severe congenital neutropenia
General
cluster-of-differentiation antigen; cluster designation antigen; CD antigen
colony-stimulating factor [CSF] receptor
glycoprotein
hematopoietin receptor superfamily
oligomerizing protein
Properties
SIZE: entity length = 836 aa
MW = 92 kD
COMPARTMENT: cellular membrane
MOTIF: signal sequence {1-24}
immunoglobulin superfamily domain {25-117}
MOTIF: N-glycosylation site {N51}
N-glycosylation site {N93}
fibronectin type III domain or F3 module {122-222}
MOTIF: N-glycosylation site {N128}
cysteine residue {C131}
MODIFICATION: cysteine residue {C142}
N-glycosylation site {N134}
cysteine residue {C142}
MODIFICATION: cysteine residue {C131}
fibronectin type III domain or F3 module {237-330}
MOTIF: cysteine residue {C248}
MODIFICATION: cysteine residue {C295}
cysteine residue {C266}
MODIFICATION: cysteine residue {C309}
cysteine residue {C295}
MODIFICATION: cysteine residue {C248}
cysteine residue {C309}
MODIFICATION: cysteine residue {C266}
WSXWS motif {318-322}
fibronectin type III domain or F3 module {334-426}
MOTIF: N-glycosylation site {N389}
fibronectin type III domain or F3 module {430-522}
MOTIF: N-glycosylation site {N474}
fibronectin type III domain or F3 module {527-618}
MOTIF: N-glycosylation site {N579}
N-glycosylation site {N610}
transmembrane domain {628-650}
box1 motif
SITE: 658-666
FOR-BINDING-OF: Janus kinase (JAK)
Database Correlations
OMIM 138971
MORBIDMAP 138971
UniProt Q99062
PFAM correlations
Entrez Gene 1441
Kegg hsa:1441
References
- UniProt :accession Q99062
- SeattleSNPs
http://pga.gs.washington.edu/data/csf3r/
- Taga T & Kishimoto T
Cytokine receptors and signal transduction
FASEB J 6:3387 1992
PMID: 1334470
- Ihle JN et al
Signaling by the cytokine receptor superfamily: JAKs and STATs
TIBS 19:222 1994
PMID: 8048164
- http://www.pathologyoutlines.com/cd100247.html
21 June 2005