glutamine-dependent NAD+ synthetase; NAD+ synthase [glutamine-hydrolyzing]; NAD+ synthetase 1 (NADSYN1)

Function: - NAD biosynthesis - homohexamer ATP + deamido-NAD(+) + L-glutamine + H₂O AMP + diphosphate + NAD+ + L-glutamate Kinetic parameters: - KM=0.49 mM for deamido-NAD+ - KM=0.089 mM for ATP - KM=1.44 mM for glutamine - KM=13.1 mM for ammonium - Vmax=0.99 nmol/min/ug enzyme deamido-NAD+ - Vmax=0.61 nmol/min/ug enzyme ATP - Vmax=0.70 nmol/min/ug enzyme glutamine Structure: - homohexamer - in C-terminal section; belongs to NAD synthetase family - contains 1 CN hydrolase domain

Properties

SIZE: MW = 79 kD entity length = 706 aa MOTIF: CN hydrolase {5-299} Ligase {325-706} MOTIF: ATP-binding site NAME: ATP-binding site SITE: 355-362 serine residue {S357}

Database Correlations

OMIM 608285 UniProt Q6IA69 PFAM correlations Entrez Gene 55191 KEGG correlations ENZYME 6.3.5.1

References

UniProt :accession Q6IA69