girdin; girders of actin filament; Akt phosphorylation enhancer; APE; hook-related protein 1; HkRP1; G alpha-interacting vesicle-associated protein (GIV, GRDN, APE, KIAA1212, CCDC88A)

Function: - interacts with the C-terminus of AKT1/PKB - enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation & kinase activity of AKT1/PKB, but does not possess kinase activity itself - phosphorylation of AKT1/PKB thereby induces phosphorylation of downstream effectors GSK3 & FOXO1A/FKHR, & regulates DNA replication & cell proliferation - phosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner - phosphorylation by AKT1/PKB is necessary for delocalization from the cell membrane & for cell migration - non-phosphorylated form interacts with PtdIns4P & weakly with PtdIns3P - essential for integrity of actin cytoskeleton & for cell migration - interacts with microtubules - interacts with actin through its C-terminal domain - required for formation of actin stress fibers & lamellipodia - role in membrane sorting in the early endosome (putative) Structure: omodimer Compartment: - membrane, cell membrane, cytoplasm, cytoplasmic vesicle. - cell projection, lamellipodium - localizes to the cell membrane through interaction with phosphoinositides Alternative splicing: named isoforms=2 Expression: expressed ubiquitously

General

cytoskeletal protein phosphoprotein

Properties

SIZE: MW = 216 kD entity length = 1871 aa COMPARTMENT: cytoplasm MOTIF: coiled coil {196-425} coiled coil {458-1385} Ser phosphorylation site {S1387} binding site SITE: 1390-1408 FOR-BINDING-OF: phosphatidylinositol Ser phosphorylation site {S1417} Thr phosphorylation site {T1559} Thr phosphorylation site {T1560} Ser phosphorylation site {S1561} Ser phosphorylation site {S1675} Ser phosphorylation site {S1702}

References

UniProt :accession Q3V6T2