Search
Geminin (GMNN)
Function:
- inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC)
- degraded during the mitotic phase of the cell cycle
- ubiquinated by anaphase-promoting complex
- its degradation at the metaphase-anaphase transition permits replication in the succeeding cell cycle
- interaction with CDT1 inhibits binding of the MCM complex to origins of replication
Structure:
- homotetramer
- belongs to the geminin family
Compartment:
- present in nucleus from S phase until mitosis
Expression:
- absent during G1 phase, accumulates during S phase, G2 phase, & M phase, & disappears at the time of the metaphase- anaphase transition
General
nuclear protein
phosphoprotein
Properties
SIZE: entity length = 209 aa
MW = 24 kD
COMPARTMENT: cell nucleus
MOTIF: Ser phosphorylation site {S63}
Ser phosphorylation site {S64}
coiled coil {94-144}
Database Correlations
OMIM 602842
UniProt O75496
Entrez Gene 51053
Kegg hsa:51053
References
- UniProt :accession O75496
- McGarry TJ & Kirschner MW.
Geminin, an inhibitor of DNA replication, is degraded during
mitosis.
Cell 93:1043-53, 1998
PMID: 9635433
- Wohlschlegel JA et al.
Inhibition of eukaryotic DNA replication by geminin binding
to Cdt1.
Science 290:2309-12, 2000
PMID: 11125146
- Lygerou Z & Nurse P.
Cell cycle. License withheld--geminin blocks DNA replication.
Science 290: 2271-3, 2000
PMID: 11188727