gamma-interferon-inducible protein 16; Ifi-16; interferon-inducible myeloid differentiation transcriptional activator (IFI16, IFNGIP1)

Function: - may function as a transcriptional repressor - could have a role in the regulation of hematopoietic cell differentiation through activation of unknown target genes - controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 & the retinoblastoma protein - may be involved in senescence of prostate epithelial cells - phosphorylated on Ser & Thr - isoform 3 seems to show a minor degree of complexcarbohydrate addition - isoform 1, isoform 2 & isoform 3 can homo- & heterodimerize - binds double-stranded DNA & cell cycle regulatory factors including p53 & Rb Structure: - belongs to the HIN-200 family - contains 1 DAPIN domain - contains 2 HIN-200 domains Compartment: nucleus Alternative splicing: named isoforms=4 Expression: - expressed in peripheral blood leukocytes, fibroblasts & lymphoid cells - present in myeloid precursors (CD34+) & throughout monocyte development, but its expression is down-regulated in erythroid & polymorphonuclear precursor cells - present in prostate, ovary & breast (at protein level) - strongly induced by gamma interferon &, to a lesser extent, by alpha interferon - in HL-60 cells, maximum induction by gamma-interferon occurs within 12 hours whereas, for alpha-interferon, only 10-fold induction was observed after 36 hours - induced in vitro by dimethylsulfoxide, retinoic acid & 1,25 dihydroxyvitamin D3

General

phosphoprotein transcriptional silencer (transcriptional repressor)

Properties

SIZE: entity length = 785 aa MW = 88 kD COMPARTMENT: cell nucleus MOTIF: lysine-rich region {1-150} MOTIF: lysine residue (SEVERAL) DAPIN domain {1-88} Ser phosphorylation site {S106} nuclear translocation signal {124-131} Ser phosphorylation site {S153} HIN-200 domain {189-389} HIN-200 domain {562-761}

Database Correlations

OMIM 147586 UniProt Q16666 PFAM correlations Entrez Gene 3428 Kegg hsa:3428

References

UniProt :accession Q16666