FYVE, RhoGEF & PH domain-containing protein 4 (actin filament-binding protein frabin, FGD1-related F-actin-binding protein, zinc finger FYVE domain-containing protein 6, FGD4, FRABP, ZFYVE6)

Function: - activates CDC42 by exchanging bound GDP for free GTP - role in regulating actin cytoskeleton & cell shape, microspike formation - activates MAPK8 Structure: - homooligomer - contains 1 DH domain (DBL-homology domain) - contains 1 FYVE-type zinc finger - contains 2 PH domains - part of protein spanning actin filament-binding domain together with DH domain & 1st PH domain necessary & sufficient for microspike formation - activation of MAPK8 requires the presence of all domains with the exception of the actin filament-binding domain Compartment: - cytoplasm, cytoskeleton, cell projection, filopodium - concentrated in filopodia & poorly detected at lamellipodia - binds along thesides of actin fibers Alternative splicing: named isoforms=3 Expression:

General

guanine nucleotide exchange factor (GDP/GTP dissociation stimulator, guanine nucleotide-releasing factor/protein, GNEF, GNDS, GDS) zinc finger FYVE domain-containing protein (ZFYVE)

Properties

SIZE: MW = 87 kD entity length = 766 aa COMPARTMENT: cytoplasm MOTIF: binding site SITE: 1-150 FOR-BINDING-OF: actin Dbl homology domain {206-393} PH domain {422-521} Zn finger Fyve-type SITE: 559-619 EFFECTOR-BOUND: Zn+2 PH domain {643-740}

Database Correlations

UniProt Q96M96 PFAM correlations

References

UniProt :accession Q96M96