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Fragile X mental retardation 1 protein; protein FMR-1; FMRP (FMR1)

Function: - RNA-binding protein that plays a role in intracellular RNA transport & in regulation of translation of target mRNAs - associated with polysomes - may play a role in the transport of mRNA from the nucleus to the cytoplasm - binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C) - RNA-binding activity is inhibited by RANBP9 - phosphorylated on several Ser (putative) - found in a RNP granule complex with IGF2BP1 - directly interacts with SMN & TDRD3 - interacts with the SMN core complex - interacts with FXR1, FXR2, IGF2BP1, NUFIP1, NUFIP2, MCRS1 & RANBP9 - interacts with CYFIP1 & CYFIP2 Structure: - homooligomer - belongs to the FMR1 family - contains 2 KH domains Compartment: cytoplasm, nucleus, nucleolus Alternative splicing: named isoforms=8; at least 12 different isoforms are produced Expression: - highest levels found in neurons, brain, testis, placenta & lymphocytes - also expressed in epithelial tissues & at very low levels in glial cells Pathology: - defects in FMR1 are the cause of: a) fragile X syndrome b) fragile X tremor/ataxia syndrome (FXTAS) - severe fragile X syndrome phenotype with Asn-304; involves sequestration of bound mRNAs in nontranslatable mRNP particles; in the absence of FMRP, these same mRNAs may be partially translated via alternate mRNPs, although perhaps abnormally localized or regulated, resulting in typical fragile X syndrome; Asn-304 mutation maps to a position within the 2nd KH domain of FMRP that is critical for stabilizing sequence-specific RNA-protein interactions; Asn-304 mutation abrogates the association of the 2nd FMRP KH domain with its target, kissing complex RNA

Related

FMR-1 gene FMR1 gene activation FMR1 gene allele 1 CGG repeats FMR1 gene allele 2 CGG repeats FMR1 gene CGG repeats FMR1 gene methylation FMR1 gene mutation

General

nuclear protein RNA-binding protein

Properties

SIZE: entity length = 632 aa MW = 71 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: KH domain SITE: 222-251 FOR-BINDING-OF: ribonucleic acid KH domain SITE: 285-314 FOR-BINDING-OF: ribonucleic acid RANBP9 interaction {419-632} MOTIF: Ser phosphorylation site {S500} binding site SITE: 534-548 FOR-BINDING-OF: ribonucleic acid

Database Correlations

OMIM correlations MORBIDMAP 309550 UniProt Q06787 PFAM correlations Entrez Gene 2332 Kegg hsa:2332

References

  1. Ross CA, McInnis MG, Margolis RL, Li SH. Genes with triplet repeats: candidate mediators of neuropsychiatric disorders. Trends Neurosci. 1993 Jul;16(7):254-60. Review. PMID: 7689767
  2. Siomi H, Siomi MC, Nussbaum RL, Dreyfuss G. The protein product of the fragile X gene, FMR1, has characteristics of an RNA-binding protein. Cell. 1993 Jul 30;74(2):291-8. PMID: 7688265
  3. UniProt :accession Q06787
  4. GeneReviews https://www.genecards.org/cgi-bin/carddisp.pl?gene=FMR1