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fecal DNA testing
Indications:
- screening for colon cancer
Clinical significance:
- more sensitive, but less specific than fecal occult blood (FOB)
- sensitivity of fecal DNA testing for detecting colorectal cancer is 52% vs 13% for FOB [1]
- sensitivity of fecal DNA testing for detecting advanced tubular adenomas is 18% vs 11% for FOB [1]
- sensitivity 92% vs. 74% (FOB) for detecting colorectal cancer [3]
- specificity 87% vs. 95% (FOB) for detecting colorectal cancer [3]
Notes:
- cost: $600 (2014)
Specific
digital protein truncation assay
fecal RT-PCR of 10 DNA markers + fecal hemoglobin
multitarget stool DNA; mt-sDNA, (Cologuard)
General
molecular diagnostic test
DNA (genetic) testing
References
- Journal Watch 25(2):13, 2005
Imperiale TF, Ransohoff DF, Itzkowitz SH, Turnbull BA, Ross ME;
Colorectal Cancer Study Group.
Fecal DNA versus fecal occult blood for colorectal-cancer
screening in an average-risk population.
N Engl J Med. 2004 Dec 23;351(26):2704-14.
PMID: 15616205
- Traverso G, Shuber A, Olsson L, Levin B, Johnson C, Hamilton SR,
Boynton K, Kinzler KW, Vogelstein B.
Detection of proximal colorectal cancers through analysis of faecal DNA.
Lancet. 2002 Feb 2;359(9304):403-4.
PMID: 11844514
- Imperiale TF et al
Multitarget Stool DNA Testing for Colorectal-Cancer Screening.
N Engl J Med. March 18, 2014
PMID: 24645800
http://www.nejm.org/doi/full/10.1056/NEJMoa1311194
- Robertson DJ and Dominitz JA
Stool DNA and Colorectal-Cancer Screening.
N Engl J Med. March 18, 2014
PMID: 24645801
http://www.nejm.org/doi/full/10.1056/NEJMe1400092
- Carethers JM
JAMA Diagnostic Test Interpretation
Stool-Based Screening Tests for Colorectal Cancer
JAMA. Published online February 17, 2023
PMID: 36800187
https://jamanetwork.com/journals/jama/fullarticle/2801813