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fatty-acid amide hydrolase 1; anandamide amidohydrolase 1; oleamide hydrolase 1 (FAAH, FAAH1)
Function:
- degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide & myristic amide to their corresponding acids, thus serving to terminate the signaling functions of these molecules
- hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates
- degrades bioactive fatty acid amides to corresponding acids
- oleamide
- endogenous cannabinoid
- anandamide
- myristamide
anandamide + H2O = arachidonic acid + ethanolamine
Oleamide + H2O = oleic acid + NH3
Inhibition:
- inhibited by O-aryl carbamates
Structure:
- homodimer (putative)
- belongs to the amidase family
Compartment:
- endomembrane system
- single-pass membrane protein
- cytoplasm, cytoskeleton
- seems to be attached to intracellular membranes & a portion of the cytoskeletal network
Expression:
- highly expressed in the brain, small intestine, pancreas, skeletal muscle & testis
- also expressed in kidney, liver, lung, placenta & prostate
Polymorphism:
- variant Thr-129 seems to be strongly associated with illegal drug use & dependence; this variant displays normal catalytic properties but an enhanced sensitivity to proteolytic degradation
Related
fatty-acid amide hydrolase
General
hydrolase
membrane protein
phosphoprotein
Properties
SIZE: entity length = 579 aa
MW = 63 kD
COMPARTMENT: cytoplasm
MOTIF: transmembrane domain {9-29}
lysine residue {K142}
binding site
SITE: 191-191
FOR-BINDING-OF: Substrate; via carbonyl oxygen
serine residue {S217}
binding site
SITE: 217-217
FOR-BINDING-OF: Substrate
Substrate binding {238-241}
MOTIF: serine residue {S241}
Tyr phosphorylation site {Y329}
Tyr phosphorylation site {Y335}
Database Correlations
OMIM 602935
MORBIDMAP 602935
UniProt O00519
Pfam PF01425
Entrez Gene 2166
Kegg hsa:2166
ENZYME 3.5.1.99
References
- UniProt :accession O00519
- NIEHS-SNPs
http://egp.gs.washington.edu/data/faah/