FACT complex

Function: 1) general chromatin factor that acts to reorganize nucleosomes 2) involved in multiple processes that require DNA as a template including mRNA elongation, DNA replication & DNA repair 3) during transcription elongation, FACT complex acts as histone chaper structure 4) facilitates passage of RNA polymerase 2 & transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes reestablishment of the nucleosome following passage of RNA polymerase 2 5) role in phosphorylation of Ser-392 of p53/TP53 via association with casein kinase 2 6) ADP-ribosylation of SUPT16H by PARP1 is induced by genotoxic stress & correlates with dissociation of FACT from chromatin Structure: - heterodimer of SSRP1 & SUPT16H

Properties

SUBUNITS: FACT complex subunit SPT16 COMPARTMENT: cell nucleus MOTIF: coiled coil {432-507} glutamate-rich region {926-1011} MOTIF: glutamate residue (SEVERAL) FACT complex subunit SSRP1 COMPARTMENT: cell nucleus MOTIF: acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl Thr phosphorylation site {T170} Tyr phosphorylation site {Y311} Ser phosphorylation site {S437} acidic region {439-496} MOTIF: acidic residue (SEVERAL) Tyr phosphorylation site {Y441} Ser phosphorylation site {S444} peptide motif {450-451} serine-rich region {497-511} MOTIF: serine residue (SEVERAL) Ser phosphorylation site {S510} lysine-rich region {512-534} MOTIF: lysine residue (SEVERAL) HMG box SITE: 547-615 MOTIF: Tyr phosphorylation site {Y554} Ser phosphorylation site {S561} lysine-rich region {623-640} MOTIF: lysine residue (SEVERAL) serine-rich region {641-709} MOTIF: serine residue (SEVERAL) Ser phosphorylation site {S657} Ser phosphorylation site {S659} Ser phosphorylation site {S667} Ser phosphorylation site {S668} Ser phosphorylation site {S671} Ser phosphorylation site {S672} Ser phosphorylation site {S673} Ser phosphorylation site {S688}

References

UniProt :accession Q9Y5B9

Components

FACT complex subunit SPT16 (facilitates chromatin transcription complex subunit SPT16, hSPT16, FACT 140 kD subunit, FACTp140, chromatin-specific transcription elongation factor 140 kD subunit, SUPT16H, FACT140, FACTP140) FACT complex subunit SSRP1; facilitates chromatin transcription complex subunit SSRP1; FACT 80 kD subunit; FACTp80; chromatin-specific transcription elongation factor 80 kD subunit; structure-specific recognition protein 1; hSSRP1; recombination signal sequence recognition protein 1; T160 (SSRP1, FACT80)