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exonuclease 5; exo V; hexo5; defects in morphology protein 1 homolog (EXO5, C1orf176, DEM1)
Function:
- single-stranded DNA (ssDNA) bidirectional exonuclease involved in DNA repair
- probably involved in DNA repair following ultraviolet (UV) irradiation & interstrand cross-links
- has both 5'-3' & 3'-5' exonuclease activities with a strong preference for 5'-ends
- acts as a sliding exonuclease that loads at ssDNA ends & then slides along the ssDNA prior to cutting
- sliding & 3'-5' exonuclease activities are abolished upon binding to the replication protein A complex that enforces 5'-directionality activity
- monomer;
- monomeric form has weak exonuclease activity
- homodimer;
- homodimeric has much higher exonuclease activity,
- could homodimerize upon DNA-binding
- interacts with the replication protein A complex
Cofactor:
- binds 1 4Fe-4S cluster
- Mg+2 (putative)
Structure: belongs to the EXO5 family
Compartment:
- nucleus. cytoplasm
- localizes to repair foci in response to DNA damage
General
exonuclease
nuclear protein
Properties
SIZE: entity length = 373 aa
MW = 42 kD
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: cofactor-binding site [92-92]
COFACTOR-BOUND: iron sulfur [FeS] cluster
cofactor-binding site [343-343]
COFACTOR-BOUND: iron sulfur [FeS] cluster
cofactor-binding site [346-346]
COFACTOR-BOUND: iron sulfur [FeS] cluster
cofactor-binding site [352-352]
COFACTOR-BOUND: iron sulfur [FeS] cluster
Database Correlations
UniProt Q9H790
Pfam PF09810
Entrez Gene 64789
Kegg hsa:64789
References
UniProt :accession Q9H790