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energy-dependent nucleolar silencing complex (eNoSC complex)
Function:
- mediates silencing of rDNA in response to intracellular energy status
- acts by recruiting histone-modifying enzymes
- able to sense the energy status of cell
- upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at Lys-9 (H3K9me2) by SUV39H1 & the formation of silent chromatin in the rDNA
Structure:
- subunits include: SIRT1, SUV39H1 & RRP8
General
molecular complex
Properties
SUBUNITS: sirtuin 1
COMPARTMENT: cell nucleus
MOTIF: alanine-rich region {54-98}
MOTIF: alanine residue (SEVERAL)
POLY-ASP {122-127}
glutamate residue {128-134} (6)
DEACETYLASE SIRTUIN-TYPE {244-498}
MOTIF: histidine residue {H363}
Zn+2-binding site
SITE: 371-371
Zn+2-binding site
SITE: 374-374
Zn+2-binding site
SITE: 395-395
Zn+2-binding site
SITE: 398-398
histone-lysine N-methyltransferase SUV39H1
COMPARTMENT: cell nucleus
MOTIF: SIRT1 interaction {1-89}
chromodomain
NAME: chromodomain
SITE: 43-101
FOR-BINDING-OF: heterochromatin
pre-SET domain {179-240}
SET domain {242-370}
Mediates interaction with MECOM {255-377}
Ser phosphorylation site {S391}
post-SET domain {396-412}
ribosomal RNA-processing protein 8
COMPARTMENT: cell nucleus
MOTIF: Ser phosphorylation site {S58}
Ser phosphorylation site {S62}
Ser phosphorylation site {S64}
glutamate-rich region {65-70}
MOTIF: glutamate residue (SEVERAL)
Ser phosphorylation site {S104}
Ser phosphorylation site {S106}
Ser phosphorylation site {S124}
Ser phosphorylation site {S126}
Ser phosphorylation site {S171}
Thr phosphorylation site {T175}
Ser phosphorylation site {S176}
Ser phosphorylation site {S223}
active site
binding site
SITE: 281-281
FOR-BINDING-OF: S-adenosylmethionine
binding site
SITE: 316-316
FOR-BINDING-OF: S-adenosylmethionine
binding site
SITE: 334-334
FOR-BINDING-OF: S-adenosylmethionine
binding site
SITE: 346-346
FOR-BINDING-OF: S-adenosylmethionine
binding site
SITE: 347-347
FOR-BINDING-OF: S-adenosylmethionine
binding site
SITE: 363-363
FOR-BINDING-OF: S-adenosylmethionine
References
UniProt :accession O43463
Components
histone-lysine N-methyltransferase SUV39H1; histone H3-K9 methyltransferase 1; H3-K9-HMTase 1; lysine N-methyltransferase 1A; position-effect variegation 3-9 homolog; suppressor of variegation 3-9 homolog 1; Su(var)3-9 homolog 1 (SUV39H1, KMT1A, SUV39H)
ribosomal RNA-processing protein 8; cerebral protein 1; nucleomethylin (RRP8, KIAA0409, NML, hucep-1)
sirtuin 1; NAD-dependent deacetylase sirtuin 1; silent mating type information regulation 2 homolog 1 (SIRT1)