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endothelin-converting enzyme 2; ECE-2 (ECE2, KIAA0604)
Function:
- converts big endothelin-1 to endothelin-1
- role in processing of various neuroendocrine peptides, including: neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, & prodynorphin-derived peptides
- may limit beta-amyloid peptide accumulation in brain
- may also have methyltransferase activity
- hydrolysis of 21-Trp-|-Val-22 bond in big endothelin to form endothelin 1
Cofactor: binds 1 Zn+2 per subunit (putative)
Kinetic parameters:
- KM=0.4 uM for big ET-1
- KM=1.4 uM for peptide E
- KM=27.4 uM for bradykinin
- KM=48.4 uM for dynorphin B
- optimum pH is 5.0-5.5; inactive at neutral pH
Structure:
- in the N-terminal section; belongs to the methyltransferase superfamily M13 family
- in the C-terminal section; belongs to the peptidase
Compartment:
- Golgi membrane
- cytoplasmic granule membrane
Alternative splicing: named isoforms=4; A-D
Expression:
- expressed in brain
Pathology:
- strongly down-regulated in inferior parietal lobe of patients with Alzheimer disease (at protein level)
General
endothelin-converting enzyme
glycoprotein
phosphoprotein
Properties
SIZE: entity length = 811 aa
MW = 91 kD
COMPARTMENT: cytoplasm
golgi
MOTIF: Methyltransferase-like region {1-160}
MOTIF: Tyr phosphorylation site {Y39}
transmembrane domain {179-199}
Endothelin-converting enzyme 2 region {200-883}
MOTIF: N-glycosylation site {N324}
N-glycosylation site {N384}
N-glycosylation site {N429}
Zn+2-binding site
SITE: 720-720
glutamate residue {E721}
Zn+2-binding site
SITE: 724-724
Zn+2-binding site
SITE: 780-780
aspartate residue {D784}
Database Correlations
OMIM 610145
UniProt P0DPD6
PFAM correlations
LOCUS-LINK correlations
KEGG correlations
ENZYME 3.4.24.71
References
- UniProt :accession P0DPD6
- UniProt PubMed refs
- PMID: 12560336