Search
endothelial differentiation-related factor 1; EDF-1; multiprotein-bridging factor 1 (MBF1, EDF1)
Function:
1) transcriptional coactivator
2) stimulates NR5A1 & ligand-dependent NR1H3/LXRA & PPARG transcriptional activities
3) enhances the DNA-binding activity of ATF1, ATF2, CREB1 & NR5A1
4) regulates nitric oxide synthase activity via sequestering calmodulin in the cytoplasm
- binding to calmodulin is regulated by Ca+2 & phosphorylation of the IQ motif
5) endothelial cell differentiation
6) hormone-induced cardiomyocyte hypertrophy
7) lipid metabolism
8) interacts with TBP & the transcription factor IID complex
- interaction with TBP is regulated by phosphorylation
9) interaacts with NR5A2, NR1H3 & PPARG.
10) binds NR5A1, ATF1, FOS & JUN via their conserved basic region
11) phosphorylated by PKA & PKC
Compartment: cytoplasm, nucleus
Alternative splicing: named isoforms=2
Expression:
- ubiquitously expressed
- expressed in brain, liver, lung, kidney, heart, pancreas, intestine, adipose tissues
- expressed in fetal tissues
Pathology:
- down-regulated by HIV type 1 protein TAT or phorbol ester treatment in endothelial cells
General
nuclear protein
phosphoprotein
Properties
SIZE: MW = 16 kD
entity length = 148 aa
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: phosphorylation site
NR5A2/PPARG/NR1H3 interaction {37-113}
MOTIF: TBP/NR5A1 interaction {69-108}
HTH CRO/C1-TYPE {81-135}
MOTIF: Q motif {81-88}
DNA-binding motif
SITE: 92-111
Database Correlations
OMIM 605107
UniProt O60869
PFAM correlations
References
UniProt :accession O60869