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endonuclease 8-like 3; DNA glycosylase FPG2; DNA glycosylase/AP lyase Neil3; endonuclease VIII-like 3; Nei-like protein 3 (NEIL3)
Function:
- DNA glycosylase activity is uncertain (controversial)
- may prefer ssDNA or partially ssDNA structures such as bubble & fork structures to dsDNA in vitro
- broad recognition spectrum, preferring FapyA & FapyG > 5-OHU, 5-PHC & 5-OHMH > Tg & 8-oxoA
- no activity on 8-oxoG
- removes damaged bases from DNA, leaving an abasic site
- the C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar & a product with a terminal 5'-phosphate
Structure:
- belongs to the FPG family
- lacks a Pro at the N-terminus which functions as an active site residue in other members of the FPG family
- contains 1 FPG-type Zn+2 finger
- contains 1 RanBP2-type Zn+2 finger
Compartment: nucleus
Expression:
- detected in thymus, pancreas & testis
- expressed at higher levels in tumor tissue than in the corresponding normal tissues except for pancreas & testis where expression is higher in normal than tumor tissue
General
DNA-binding protein
DNA glycosylase
zinc finger protein
Properties
SIZE: entity length = 605 aa
MW = 68 kD
COMPARTMENT: cell nucleus
MOTIF: valine residue {V2}
DNA-binding motif
SITE: 192-192
Zinc finger
NAME: Zinc finger
SITE: 247-281
EFFECTOR-BOUND: Zn+2
MOTIF: DNA-binding motif
SITE: 271-271
Zinc finger
NAME: Zinc finger
SITE: 317-346
EFFECTOR-BOUND: Zn+2
Database Correlations
OMIM 608934
UniProt Q8TAT5
PFAM correlations
Entrez Gene 55247
Kegg hsa:55247
ENZYME 4.2.99.18
References
- UniProt :accession Q8TAT5
- NIEHS-SNPs
http://egp.gs.washington.edu/data/neil3/