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endonuclease 8-like 2; DNA glycosylase/AP lyase Neil2; DNA-(apurinic or apyrimidinic site) lyase Neil2; endonuclease VIII-like 2; Nei homolog 2; NEH2; Nei-like protein 2 (NEIL2)
Function:
1) involved in base excision repair of DNA damaged by oxidation or by mutagenic agents
2) DNA glycosylase activity towards 5-hydroxyuracil & other oxidized derivatives of cytosine
3) preference for mismatched double stranded DNA (DNA bubbles)
4) low or no DNA glycosylase activity towards: thymine glycol, 2-hydroxyadenine, hypoxanthine, 8-oxoguanine
5) AP (apurinic/apyrimidinic) lyase activity
- introduces nicks in DNA
6) cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of removed base
7) removes damaged bases from DNA, leaving an abasic site.
8) acetylation of Lys-49 leads to loss of DNA nicking activity
9) binds EP300
Structure:
- the Zn+2-finger domain is important for DNA binding
- belongs to the FPG family
- contains 1 FPG-type Zn+2 finger
Compartment: nucleus
Alternative splicing: named isoforms=3
Expression:
- expressed in testis, skeletal muscle, heart, brain, placenta, lung, pancreas, kidney, liver
General
DNA-binding protein
DNA glycosylase
lyase
zinc finger protein
Properties
SIZE: entity length = 332 aa
MW = 37 kD
COMPARTMENT: cell nucleus
MOTIF: proline residue {P2}
glutamate residue {E3}
lysine residue {K50}
DNA-binding motif
SITE: 231-231
Zinc finger
NAME: Zinc finger
SITE: 284-320
EFFECTOR-BOUND: Zn+2
MOTIF: arginine residue {R310}
Database Correlations
OMIM 608933
UniProt Q969S2
Pfam PF06831
ENZYME 4.2.99.18
References
- UniProt :accession Q969S2
- NIEHS-SNPs
http://egp.gs.washington.edu/data/neil2/