Search
CD73 (ecto-5'nucleotidase, 5-prime-ribonucleotide phosphohydrolase, NT5E)
Function:
- catalyzes conversion of purine 5-prime mononucleotides to nucleosides at neutral pH
- hydrolyzes extracellular nucleotides into membrane permeable nucleosides
Cofactor: Zn+2
Compartment: cell membrane, lipid-anchor, GPI-anchor
Expression:
- B-cell & T-cell subsets
- endothelial cells
- follicular dendritic cells
- epithelial cells
Pathology:
- decreased activity in B cell CLL
- high levels poor prognostic factor in leukemias/lymphomas
Note:
- marker of lymphocytic differentiation
- other forms of 5'nucleotidases exist in cytoplasm & lysosomes & can be distinguished by substrate affinities
General
5'nucleotidase (5'-ribonucleotide phosphohydrolase)
cluster-of-differentiation antigen; cluster designation antigen; CD antigen
glypiated protein
oligomerizing protein
Properties
SIZE: entity length = 574 aa
MW = 63 kD
COMPARTMENT: cytoplasm
lysosome
plasma membrane
MOTIF: signal sequence {1-26}
Zn+2-binding site
SITE: 36-36
Zn+2-binding site
SITE: 38-38
N-glycosylation site {N53}
Zn+2-binding site
SITE: 85-85
Zn+2-binding site
SITE: 117-117
histidine residue {118}
aspartate residue {121}
Zn+2-binding site
SITE: 220-220
Zn+2-binding site
SITE: 243-243
N-glycosylation site {N311}
N-glycosylation site {N333}
N-glycosylation site {N403}
binding site
SITE: 417-417
FOR-BINDING-OF: Substrate
Substrate binding {500-506}
glycosyl phosphatidylinositol [GPI] membrane anchor {N549}
Database Correlations
OMIM 129190
UniProt P21589
PFAM correlations
Kegg hsa:4907
ENZYME 3.1.3.5
References
- Tietz Fundamentals of Clinical Chemistry 3rd ed, WB
Saunders, 1987 pg 412
- OMIM :accession 129190
- UniProt :accession P21589
- http://www.pathologyoutlines.com/cdmarkers.html
20 January 2003