ecotropic viral integration site 5 protein homolog; EVI-5; neuroblastoma stage 4S gene protein (EVI5, NB4S)

Function: 1) regulates cell cycle progression 2) stabilizes FBXO5 protein 3) promotes cyclin-A accumulation during interphase 4) role in cytokinesis (putative) 5) interacts with alpha-tubulin, gamma-tubulin, FBXO5, chromosome passenger complex 6) down-regulated during mitosis through proteasomal degradation 7) depletion of EVI5 by RNAi causes cell cycle arrest & mitotic abnormalities 8) phosphorylation by PLK1 (putative) may be required for degradation during mitosis 9) ubiquitinated, degradation during prophase is ubiquitin-dependent Structure: dimeric & monomeric Compartment: - nucleus, centrosome - associates with the mitotic spindle through anaphase & remains within midzone & midbody until completion of cytokinesis Expression: - expressed in various cell lines & in a wide range of tissues including brain & adrenal Pathology: - chromosomal aberration involving EVI5 associated with a case of neuroblastoma, translocation t(1;10)(p22;q21), forms a EVI5-TRNG10 fusion protein; TRNG10 is probable structural transcript, normally not translated

General

nuclear protein phosphoprotein

Properties

SIZE: MW = 93 kD entity length = 810 aa COMPARTMENT: cell nucleus MOTIF: binding site SITE: 1-483 FOR-BINDING-OF: tubulin MOTIF: serine-rich region {28-128} MOTIF: serine residue (SEVERAL) dimerization {128-693} MOTIF: rab-GAP domain NAME: rab-GAP domain SITE: 163-348 binding site SITE: 377-810 FOR-BINDING-OF: centrosome MOTIF: coiled coil {406-716} Ser phosphorylation site {S477} binding site SITE: 487-810 FOR-BINDING-OF: serine/threonine protein kinase 12 inner centromere protein

Database Correlations

OMIM 602942 UniProt O60447

References

UniProt :accession O60447