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transcription factor DP-1; E2F dimerization partner 1; DRTF polypeptide-1; TFDP1 (DP1, DRTF1)
Function:
- can stimulate E2F-dependent transcription
- binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication
- component of the E2F/DP transcription factor complex [1,2]
- forms heterodimers with E2F family members
- part of the E2F6.com-1 complex in G0 phase
- component of the DREAM complex
- phosphorylation by E2F-1-bound cyclin A-CDK2, in the S phase,
- inhibits E2F-mediated DNA binding & transactivation
Structure: belongs to the E2F/DP family
Compartment: nucleus
Expression:
1) constitutively expressed throughout the cell cycle
2) down-regulated during differentiation
3) expressed in muscle > brain, placenta, liver & kidney > lung & pancreas
4) not detected in heart
Note:
- DRTF1 was initially characterized as a site-specific DNA-binding transcription factor down-regulated during differentiation of F9 embryonal carcinoma cells
Interactions
molecular events
General
DNA-binding protein
transcription factor (TF)
Properties
SIZE: entity length = 410 aa
MW = 45 kD
COMPARTMENT: cell nucleus
MOTIF: Ser phosphorylation site {S23}
DNA-binding motif
SITE: 113-195
MOTIF: DEF box {161-195}
Ser phosphorylation site {S183}
Dimerization {204-277}
binding site
SITE: 211-327
FOR-BINDING-OF: E2F
MOTIF: DCB1 {214-246}
proline-rich region
SITE: 250-253
MOTIF: proline residue (SEVERAL)
DCB2 {259-315}
acidic region {394-410}
MOTIF: acidic residue (SEVERAL)
References
- Girling R et al
A new component of the transcription factor DRTF1/E2F
Nature 362:83 1993
PMID: 8446173
- Xu M et al
Cyclin A/CDK2 binds directly to E2F-1 and inhibits the
DNA-binding activity of E2F-1/DP-1 by phosphorylation.
Mol Cell Biol 14:8420 1994
PMID: 7969176
- Bandara LR et al
Functional synergy between DP-1 and E2F-1 in the cell cycle-
regulating transcription factor DRTF1/E2F.
EMBO J 12:4317 1993
PMID: 8223441
- Entrez Gene :accession 7027
- UniProt :accession Q14186
Component-of
molecular complex
Databases & Figures
OMIM 189902
UniProt Q14186
PFAM correlations
LOCUS-LINK correlations
Kegg hsa:7027
Figures/diagrams/slides/tables related to transcription factor DP-1