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DRTF1/E2F (DP1/E2F)
Function:
- role in the control of cell-cycle progression from G1 to S phase
- appears to mediate both cell proliferation & apoptosis
- binds to E2F site
- the complex can interact with hypophosphorylated RB1 & related proteins (RBL1 & RBL2) that inhibit the E2F transactivation domain
- this repression involves recruitment of histone deacetylase
- during the cell cycle, from mid-to-late G1 phase, RB family members become phosphorylated, detach from DRTF1/E2F to render E2F transcriptionally active
- viral oncoproteins, notably E1A, T-antigen & HPV E7, are capable of sequestering RB protein, thus releasing the active complex
- RB1 inhibits the transcriptional activity of DRTF1/E2F during cell cycle progression
- when mutant RB1 is present, DRTF1/E2F is deregulated
- binding to RB1 & RB-related protein p107 inhibits DNA-binding activity of DRTF1/E2F
- E2F/DP transactivation can be mediated by several cofactors including TBP, TFIIH, MDM2 & CBP
General
DNA-binding protein
transcription factor complex
Figures/Diagrams
p53 -> cell cycle
Properties
COMPARTMENT: cell nucleus
MOTIF: DNA-binding motif
SUBUNITS: transcription factor DP-1
MOTIF: Ser phosphorylation site {S23}
DNA-binding motif
SITE: 113-195
MOTIF: DEF box {161-195}
Ser phosphorylation site {S183}
domain {84-204}
MOTIF: DNA-binding motif
binding site
EFFECTOR-BOUND: E2F
BOUND-VIA: binding site
binding site
SITE: 211-327
FOR-BINDING-OF: E2F
MOTIF: DCB1 {214-246}
proline-rich region
SITE: 250-253
MOTIF: proline residue (SEVERAL)
DCB2 {259-315}
acidic region {394-410}
MOTIF: acidic residue (SEVERAL)
E2F
MOTIF: binding site
SITE: N-TERMINAL
FOR-BINDING-OF: CDK2/cyclin A complex
domain {CENTRAL}
MOTIF: DNA-binding motif
binding site
EFFECTOR-BOUND: transcription factor DP-1
BOUND-VIA: binding site
phosphorylation site
binding site
SITE: C-TERMINAL
FOR-BINDING-OF: retinoblastoma-associated protein
References
- Girling et al Nature 362:83 1993
- UniProt :accession Q14186
Components
E2F
transcription factor DP-1; E2F dimerization partner 1; DRTF polypeptide-1; TFDP1 (DP1, DRTF1)