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DNA replication factor Cdt1; double parked homolog; DUP (CDT1)
Function:
1) cooperates with CDC6 to promote loading of mini-chromosome maintenance complex onto chromatin to form pre-replication complex necessary to initiate DNA replication
2) binds DNA (sequence-, strand-, & conformation-independent)
3) potential oncogene protein
4) interaction with geminin (GMNN) inhibits both binding of MCM complex to origins of replication & DNA-binding activity which may prevent inappropriate replication origin firing
5) ubiquitinated, leading to its subsequent degradation
6) interaction with GMNN protects it against ubiquitination
7) phosphorylation by cyclin A-dependent kinases resulting in binding of CDT1 to F-box protein SKP2 & subsequent degradation
8) binding to GMNN is not affected by phosphorylation
Structure:
- belongs to the Cdt1 family
Compartment:
- nucleus
Expression:
1) present during G1 phase & early S phase of cell cycle
2) degraded during the late S phase, G2 phase, & M phase
General
nuclear protein
phosphoprotein
Properties
SIZE: entity length = 546 aa
MW = 60 kD
COMPARTMENT: cell nucleus
MOTIF: Thr phosphorylation site {T29}
Ser phosphorylation site {S31}
Cyclin-binding {68-70}
GMNN interaction {150-190}
Ser phosphorylation site {S318}
Ser phosphorylation site {S372}
Ser phosphorylation site {S394}
Database Correlations
OMIM 605525
UniProt Q9H211
Pfam PF08839
Entrez Gene 81620
Kegg hsa:81620
References
- UniProt :accession Q9H211
- Wohlschlegel JA, Dwyer BT, Dhar SK, Cvetic C, Walter JC, Dutta A.
Inhibition of eukaryotic DNA replication by geminin binding to Cdt1.
Science. 2000 Dec 22;290(5500):2309-12.
PMID: 11125146
Component-of
pre-replication complex