DEP domain-containing protein 2 (phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2, PtdIns(3,4,5)-dependent Rac exchanger 2, P-Rex2, DEPDC2, PREX2)

Function: - RAC1 guanine nucleotide exchange factor (GEF) - activates Rac proteins by exchanging bound GDP for free GTP - synergistically activated by PIP3 & the beta gamma subunits of heterotrimeric G protein - mediates activation of RAC1 in a PI3K-dependent manner - role in Rac signaling - acts downstream of both G protein-coupled receptors & phosphoinositide 3-kinase - interacts with RAC1 Structure: - PH domain confers substrate specificity & recognition able to discriminate between RAC1, RHOA, CDC42 - DH domain alone was unable to confer substrate specificity & recognition - contains 2 DEP domains - contains 1 DH domain (DBL-homology domain) - contains 2 PDZ domain (DHR domains) - contains 1 PH domain Alternative splicing: named isoforms=3 Expression: - isoform 1: highly expressed in skeletal muscle, heart, placenta, absent from peripheral blood leukocytes - isoform 2 is expressed in skeletal muscle, kidney, small intestine, placenta - isoform 3 is expressed in the heart

General

DEP domain-containing protein 5 (DEPDC) guanine nucleotide exchange factor (GDP/GTP dissociation stimulator, guanine nucleotide-releasing factor/protein, GNEF, GNDS, GDS)

Properties

SIZE: MW = 183 kD entity length = 1606 aa MOTIF: Dbl homology domain {23-214} PH domain {245-361} DEP domain {390-464} DEP domain {491-566} PDZ domain NAME: PDZ domain SITE: 592-671 PDZ domain NAME: PDZ domain SITE: 677-754

Database Correlations

UniProt Q70Z35 PFAM correlations

References

UniProt :accession Q70Z35