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deoxyribonuclease-2-alpha; acid Dnase; deoxyribonuclease II alpha; Dnase II alpha; lysosomal Dnase II; R31240-2 (DNASE2, DNASE2A, DNL2)
Function:
- hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA
- plays a major role in the degradation of nuclear DNA in cellular apoptosis during development
- necessary for proper fetal development & for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells
- endonucleolytic cleavage to nucleoside 3'-phosphates & 3'-phosphooligonucleotide end-products
- glycosylated. mutations that eliminate N-glycosylation sites reduce activity, but enzymatic deglycosylation has no effect
Structure:
- belongs to the Dnase II family
Compartment: lysosome
Notes:
- not required for the generation of the characteristic DNA fragmentation observed in apoptotic cells
- active in degradation of DNA from necrotic cells (putative)
General
deoxyribonuclease (DNase)
glycoprotein
Properties
SIZE: entity length = 360 aa
MW = 40 kD
COMPARTMENT: lysosome
MOTIF: signal sequence {1-18}
cysteine residue {C19}
MODIFICATION: cysteine residue {C159}
N-glycosylation site {N86}
cysteine residue {C159}
MODIFICATION: cysteine residue {C19}
N-glycosylation site {N212}
N-glycosylation site {N266}
cysteine residue {C267}
MODIFICATION: cysteine residue {C347}
N-glycosylation site {N290}
histidine residue {H295}
cysteine residue {C308}
MODIFICATION: cysteine residue {C327}
cysteine residue {C327}
MODIFICATION: cysteine residue {C308}
cysteine residue {C347}
MODIFICATION: cysteine residue {C267}
Database Correlations
OMIM 126350
UniProt O00115
Pfam PF03265
Entrez Gene 1777
Kegg hsa:1777
References
- UniProt :accession O00115
- OMIM :accession 126350