denticleless protein homolog (Lethal(2) denticleless protein homolog, Ddb1- & Cul4-associated factor 2, retinoic-acid regulated nuclear matrix-associated protein, DTL, CDT2, CDW1, DCAF2, L2DTL, RAMP)

Function: - required for CDT1 proteolysis in response to DNA damage through the CUL4-DDB1 E3 ubiquitin-protein ligase - role in cell cycle regulation of DNA replication (putative) - substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex - probable part of a cullin-RING E3 protein ligase complex - interacts with CUL4B & DDB1 - phosphorylated upon DNA damage, probably by ATM or ATR Structure: - belongs to the WD repeat cdt2 family. - contains 7 WD repeats Compartment: - nucleus, nucleoplasmic side, cytoplasm - nuclear matrix-associated - translocates from interphase nucleus to the metaphase cytoplasm during mitosis Alternative splicing: named isoforms=2 Expression: - expressed in placenta & testis - very low expression in skeletal muscle - detected in all hematopoietic tissues examined - highest expression in thymus & bone marrow - low level detected in the spleen & lymph node - barely detectable level in the peripheral leukocyte - retinoic acid down-regulates expression in NT2 cells - expressed in all fetal tissues examined, including brain, lung, liver, & kidney Pathology: - role in cell proliferation of NT2 embryonal carcinoma cells

General

nuclear protein phosphoprotein WD repeat protein family

Properties

SIZE: MW = 79 kD entity length = 730 aa COMPARTMENT: cytoplasm cell nucleus MOTIF: WD repeat {47-89} WD repeat {96-135} WD repeat {138-178} MOTIF: DDB1-binding {168-171} nuclear translocation signal {197-203} WD repeat {214-253} MOTIF: DDB1-binding {243-246} WD repeat {267-308} WD repeat {313-354} WD repeat {358-398} Ser phosphorylation site {S426} Thr phosphorylation site {T516} Ser phosphorylation site {S599} Ser phosphorylation site {S717}

Database Correlations

UniProt Q9NZJ0 Pfam PF00400

References

UniProt :accession Q9NZJ0