Search
dedicator of cytokinesis protein 4 (DOCK4, KIAA0716)
Function:
1) regulation of adherens junction between cells
2) guanine nucleotide exchange factor (GEF)
- activates Rap1 small GTPase by exchanging bound GDP for free GTP
3) interacts with the SH3 domain of CRK
4) interacts with nucleotide-free Rap1
5) interacts with FASLG
6) interacts with ELMO2 & EPHA2
7) mediates activation of RAC1 by EPHA2
Structure:
- DHR-2 domain probably mediates the GEF activity
- belongs to the DOCK family
- contains 1 DHR-1 domain
- contains 1 DHR-2 domain
- contains 1 SH3 domain
Compartment:
- endomembrane system, peripheral membrane
- cytoplasm
Alternative splicing: named isoforms=4
Expression:
- widely expressed at low level
- highly expressed in skeletal muscle, prostate, ovary
Pathology:
- defects in DOCK4 associated with some cancers thought due to lack of Rap1 activation
- DOCK4 (wild-type protein) suppresses tumor invasion in osteosarcoma mouse cell lines
General
dedicator of cytokinesis protein (DOCK)
phosphoprotein
Properties
SIZE: MW = 225 kD
entity length = 1966 aa
COMPARTMENT: cytoplasm
MOTIF: src homology 3 [SH3] domain
SITE: 6-67
Tyr phosphorylation site {Y167}
DHR-1 {401-574}
DHR-2 {1190-1596}
Ser phosphorylation site {S1614}
Ser phosphorylation site {S1618}
Ser phosphorylation site {S1620}
serine-rich region {1642-1863}
MOTIF: serine residue (SEVERAL)
SH3-binding site
NAME: SH3-binding site
SITE: 1788-1794
Tyr phosphorylation site {Y1831}
proline-rich region
SITE: 1875-1960
MOTIF: proline residue (SEVERAL)
Database Correlations
OMIM 607679
UniProt Q8N1I0
PFAM correlations
Entrez Gene 9732
Kegg hsa:9732
References
UniProt :accession Q8N1I0