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Decapping & exoribonuclease protein; DXO; dom-3 homolog Z (DXO, DOM3L, DOM3Z, NG6)
Function:
- ribonuclease that specifically degrades pre-mRNAs with a defective 5' end cap & is part of a pre-mRNA capping quality control
- has decapping, pyrophosphohydrolase & 5'-3' exonuclease activities
- has decapping activity toward incomplete 5' end cap mRNAs such as unmethylated 5' end-capped RNA to release GpppN & 5' end monophosphate RNA*
- the 5' end monophosphate RNA is then degraded by the 5'-3' exoribonuclease activity, enabling this enzyme to decap & degrade incompletely capped mRNAs
- also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5' end triphosphate to release pyrophosphates (putative)
Cofactor: binds 2 Mg+2 (putative)
* this is in contrast to canonical decapping enzymes DCP2 & NUDT16, which release m(7)pppG (m(7)GDP)
Structure: belongs to the DXO/dom3Z family
Compartment: nucleus
Expression: ubiquitously expressed
General
exoribonuclease
nuclear protein
Properties
SIZE: entity length = 396 aa
MW = 45 kD
COMPARTMENT: cell nucleus
MOTIF: binding site
SITE: 58-58
FOR-BINDING-OF: Substrate
binding site
SITE: 101-101
FOR-BINDING-OF: Substrate
binding site
SITE: 132-132
FOR-BINDING-OF: Substrate
Mg+2-binding site
SITE: 192-192
binding site
SITE: 217-217
FOR-BINDING-OF: Substrate
Mg+2-binding site
SITE: 234-234
MOTIF: binding site
SITE: 234-234
FOR-BINDING-OF: Substrate
Mg+2-binding site
SITE: 236-236
Mg+2-binding site
SITE: 253-253
Mg+2-binding site
SITE: 254-254
binding site
SITE: 280-280
FOR-BINDING-OF: Substrate
Database Correlations
OMIM 605996
UniProt O77932
Pfam PF08652
Entrez Gene 1797
Kegg hsa:1797
References
UniProt :accession O77932