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death-associated protein kinase 3; DAP kinase 3; DAP-like kinase; Dlk; ZIP-kinase (DAPK3, ZIPK)
Function:
- serine/threonine kinase
- acts as a positive regulator of apoptosis
- phosphorylates histone H3 on Thr-11 at centromeres during mitosis
- regulates myosin light chain phosphatase via phosphorylation of MYPT1 thus regulating assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction & neurite outgrowth
- phosphorylated on Thr-265 & Thr-299 by ROCK1 which activates the kinase
- autophosphorylated
- homodimer or forms heterodimers with ATF4; both interactions require an intact leucine zipper domain
- oligomerization is required for full enzymatic activity
- also binds to DAXX & PAWR, possibly in a ternary complex which plays a role in caspase activation
- interacts with AATF & CDC5L
Cofactor: Mg+2
Structure:
- belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, DAP kinase subfamily
- contains 1 protein kinase domain
Compartment:
- nucleus, cytoplasm (putative)
- relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (putative)
- associates to centromeres from prophase to anaphase
General
nuclear protein
pro apoptotic protein
serine/threonine kinase
Properties
SIZE: entity length = 454 aa
MW = 53 kD
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: kinase domain
SITE: 13-275
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 19-27
ATP-binding site
NAME: ATP-binding site
SITE: 42-42
aspartate residue {D139}
Thr phosphorylation site {T180}
Thr phosphorylation site {T265}
Thr phosphorylation site {T299}
CDC5L interaction {395-454}
STATE: active state
Database Correlations
OMIM 603289
UniProt O43293
Pfam PF00069
Entrez Gene 1613
Kegg hsa:1613
ENZYME 2.7.11.1
References
- UniProt :accession O43293
- OMIM :accession 603289