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E3 SUMO-protein ligase PIAS1; DEAD/H box-binding protein 1; Gu-binding protein; GBP; protein inhibitor of activated STAT protein 1; RNA helicase II-binding protein (PIAS1, DDXBP1)
Function:
- E3-type small ubiquitin-like modifier (SUMO) ligase
- stabilizes interaction between UBE2I & substrate
- SUMO-tethering factor
- role in transcriptional coregulation in cellular pathways
a) STAT pathway
b) p53 pathway
c) steroid hormone signaling pathway
- effects of transcriptional coregulation may be transactivation or silencing, depending upon context
- protein modification; protein sumoylation
- sumoylated
- binds SUMO1 & UBE2I
- interacts with AR, AXIN1, CSRP2, JUN, MDM2, NCOA2, TP53, RNA helicase 2 & STAT1 dimers, following IFN-alpha stimulation
- interacts with SP3, preferentially when SUMO-modified
- preferentially binds AT-rich DNA sequences, known as matrix attachment regions or scaffold attachment regions (putative)
- interacts with PLAG1
- interacts with KLF8; interaction results in SUMO ligation & repression of KLF8 transcriptional activity & of its cell cycle progression into G1 phase
- interacts with SATB2
Structure:
- the LXXLL motif is a transcriptional coregulator signature
- the SP-RING-type domain is required for promoting EKLF sumoylation (putative)
- belongs to the PIAS family
- contains 1 PINIT domain
- contains 1 SAP domain
- contains 1 SP-RING-type Zn+2 finger
Compartment:
- nuclear speckle
- interaction with CSRP2 may induce a partial redistribution along the cytoskeleton
Expression:
- expressed in numerous tissues
- highest expression in testis
Related
DEAD/H ATP helicase or DEAD/H box
interferon-gamma activated factor; gamma-activated factor; signal transducer & activator of transcription-1 (STAT1)
General
nuclear protein
phosphoprotein
ring finger protein
Properties
SIZE: entity length = 651 aa
MW = 72 kD
COMPARTMENT: cell nucleus
MOTIF: SAP {11-45}
MOTIF: LXXLL {19-23}
nuclear translocation signal {56-64}
PINIT {124-288}
RING-finger {320-397}
MOTIF: nuclear translocation signal {368-380}
EFFECTOR-BOUND: Zn+2
FOR-BINDING-OF: DNA motif
binding site
SITE: 462-473
FOR-BINDING-OF: small ubiquitin-related modifier 1
MOTIF: Ser phosphorylation site {S466}
Ser phosphorylation site {S467}
Ser phosphorylation site {S468}
Ser phosphorylation site {S483}
Ser phosphorylation site {S485}
Thr phosphorylation site {T487}
Ser phosphorylation site {S503}
Ser phosphorylation site {S510}
4 X 4 AA repeats of N-T-S-L {520-615}
MOTIF: consensus repeat {520-523}
Ser phosphorylation site {S522}
consensus repeat {557-560}
serine-rich region {577-634}
MOTIF: serine residue (SEVERAL)
consensus repeat {598-601}
consensus repeat {612-615}
Database Correlations
OMIM 603566
UniProt O75925
Pfam PF02891
Entrez Gene 8554
Kegg hsa:8554
References
UniProt :accession O75925