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cytomegalovirus DNA

Indications: - diagnosis of cytomegalovirus (CMV) infection - newborn screening [2] Specimen: 1) body fluids a) whole blood (ACD or EDTA) b) serum c) urine d) saliva [7] 2) tissue 3) store whole blood at 4 degrees C 4) store tissue at -20 degrees C or below Procedure: - polymerase chain reaction - FDA approves COBAS AmpliPrep/COBAS TaqMan CMV test to meausure plasma CMV viral in patients with organ transplant July 2012 [4] Notes: - optimal PCR conditions utilize primers to conserved region of the CMV major immediate-early gene - a quantitative PCR protocol has been described

Related

cytomegalovirus (CMV, human herpesvirus 5, HHV5)

General

infectious agent nucleic acid

References

  1. Clinical Guide to Laboratory Tests, 3rd ed. Teitz ed., W.B. Saunders, 1995
  2. Boppana SB et al. Saliva polymerase-chain-reaction assay for cytomegalovirus screening in newborns. N Engl J Med 2011 Jun 2; 364:2111 PMID: 21631323
  3. FDA News Release: July 5, 2012 FDA approves first DNA test to help manage CMV infection in organ transplant patients http://www.fda.gov/NewsEvents/Newsroom/PressAnnouncements/ucm310691.htm
  4. Panel of 25 tests Laboratory Test Directory ARUP: 51813
  5. Cytomegalovirus by PCR, Whole Bld/Bn Mrw Laboratory Test Directory ARUP: 60031
  6. Cytomegalovirus Detection, PCR Laboratory Test Directory ARUP: 60040
  7. FDA News Release. Nov 30, 2018 FDA authorizes first test to aid in detecting a type of herpes virus in newborns called cytomegalovirus. https://www.fda.gov/NewsEvents/Newsroom/PressAnnouncements/ucm627310.htm