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crossover junction endonuclease MUS81; Holliday junction resolvase Mus81 (MUS81)
Function:
- interacts with EME1 & EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick
- typical substrates include 3'-flap structures, replication forks & nicked Holliday junctions
- may be required in mitosis for the processing of stalled or collapsed replication forks
- may self-associate
- interacts with EME1, EME2 & CHEK2
- interacts with BLM
- interaction may stimulate endonuclease activity of MUS81
- interacts with SLX4/BTBD12
- interaction links the MUS81-EME1 complex to SLX4, which may coordinate the action of the structure-specific endonuclease during DNA repair
- interacts with DCLRE1B/apollo
Cofactor: Mg+2
Structure:
- belongs to the XPF family
- contains 1 ERCC4 domain
Compartment:
- nucleus, nucleolus.
- recruited to foci of DNA damage in S-phase cells
Expression:
- widely expressed
- expressed in S phase & G2 phase
- induced by in cells treated with agents that damage DNA or block replication, independent of transcription (putative)
General
endodeoxyribonuclease
nuclear protein
phosphoprotein
Properties
SIZE: entity length = 551 aa
MW = 61 kD
COMPARTMENT: cell nucleus
MOTIF: Ser phosphorylation site {S95}
BLM interaction {125-244}
ERCC4 {270-372}
Database Correlations
OMIM 606591
UniProt Q96NY9
Pfam PF02732
Entrez Gene 80198
Kegg hsa:80198
ENZYME 3.1.22.
References
UniProt :accession Q96NY9