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crosslinked N-telopeptide in urine; urine pyridinolone & deoxypyridinolone (OsteoMark)

Urinary marker of bone resorption. Reference interval: 1) male: 23-110 NTx* 2) female: 13-96 NTx * NTx = umol bone collagen equivalents (BCE)/mol of creatinine Clinical significance: - cross-linked N-telopeptides are formed by collagenase digestion of bone-mineral matrix - cross-linked N-telopeptides are excreted in the urine & appear to be specific markers of bone resorption - the assay is especially useful for management of bone loss in osteoporosis* * Probability of a decrease in bone mineral density with NTx NTx Probability of decrease 18-38 1.4 p=0.28 38-51 2.4 p=0.03 51-67 3.8 p=0.0006 67-188 17.3 p=0.0001 Increases: -> clinical disorders a) Paget's disease b) hyperparathyroidism c) osteoporosis Method: 1) ELISA 2) assays measure N-telopeptide fragments of collagen (non-helical amino-terminal fragments) 3) these fragments contain both pyridinolone & deoxypyridinolone 4) assays are calibrated using a standard of bacterial collagenase-digested human bone collagen 5) results are expressed as equivalent moles of bone collagen type 1 normalized to creatinine Specimen: 1) urine (2 hour) 2) collect after 8 hour fast 3) store frozen

General

collagen crosslinks urine chemistry test

References

  1. Medical Knowledge Self Assessment Program (MKSAP) 11, American College of Physicians, Philadelphia 1998
  2. Clinical Guide to Laboratory Tests, 3rd ed. Teitz ed., W.B. Saunders, 1995
  3. Teitz Textbook of Clinical Chemistry and Molecular Diagnostics, 4th edition, Burtis CA, Ashwood ER, Burns DE (eds), Elsevier, St Louis, 2006, pg 1939
  4. Panel of 4 tests Laboratory Test Directory ARUP: 70062
  5. N-Telopeptide, Cross-Linked, Serum Laboratory Test Directory ARUP: 70500

Component-of

crosslinked N-telopeptide/creatinine in urine